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Literature summary extracted from

  • Yang, J.; Nie, Q.
    Engineering Escherichia coli to convert acetic acid to beta-caryophyllene (2016), Microb. Cell Fact., 15, 74 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.57 gene QHS1, the optimized QHS1 gene is expressed in Escherichia coli strain YJM63 and BL21(DE3), coexpression with genes ACS, GPPS2, and AACS to construct a beta-caryophyllene overroduction strain Artemisia annua

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.57 additional information in a robustly acetate-utilizing Escherichia coli strain, acetyl-CoA synthase (ACS), Artemisia annua beta-caryophyllene synthase (QHS1) and geranyl diphosphate synthase (GPPS2) are co-expressed to establish the engineered strain converting acetic acid to beta-caryophyllene. To further enhance beta-caryophyllene production from acetic acid, the heterologous mevalonate pathway is introduced into the cells. Acetoacetyl-CoA synthase (AACS) is also expressed in the cells to increase the precursor acetoacetyl-CoA and accordingly resulted in the increase of beta-caryophyllene. The final genetically modified strain, YJM67, accumulates the production of biomass and beta-caryophyllene up to 12.6 and 1.05 g/l during 72 h, respectively, with a specific productivity of 1.15 mg/h/g dry cells, and the conversion efficiency of acetic acid to beta-caryophyllene (gram to gram) reaching 2.1%. The yield of beta-caryophyllene on acetic acid of this strain also reaches about 5.6 % of the theoretical yield. A pH-coupled HAc fed-batch fermentation in large scale production of beta-caryophyllene Artemisia annua

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.57 (2E,6E)-farnesyl diphosphate Artemisia annua stereochemistry not specified in the publication beta-caryophyllene + diphosphate
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 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.57 Artemisia annua Q8SA63
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.57 (2E,6E)-farnesyl diphosphate stereochemistry not specified in the publication Artemisia annua beta-caryophyllene + diphosphate
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 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.57 beta-caryophyllene synthase
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 Artemisia annua
4.2.3.57 QHS1
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 Artemisia annua

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.57 30
-
 in vivo assay at Artemisia annua