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Literature summary extracted from

  • Patrikainen, P.; Carbonell, V.; Thiel, K.; Aro, E.M.; Kallio, P.
    Comparison of orthologous cyanobacterial aldehyde deformylating oxygenases in the production of volatile C3-C7 alkanes in engineered E. coli (2017), Metab. Eng. Commun., 5, 9-18 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.5 recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Prochlorococcus marinus), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis Prochlorococcus marinus
4.1.99.5 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechococcus sp. RS9917), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis Synechococcus sp. RS9917
4.1.99.5 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase (from Synechocystis sp. PCC 6803), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis Synechocystis sp. PCC 6803
4.1.99.5 recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr, simultaneous coexpression with four enzymes required in the alkane pathway: aldehyde deformylating oxygenase, ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), expression analysis Nostoc punctiforme

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.5 A134F site-directed mutagenesis, the mutant shows slightly increased activity compared to wild-type Prochlorococcus marinus
4.1.99.5 additional information installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Nostoc punctiforme ), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo Nostoc punctiforme
4.1.99.5 additional information installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Prochlorococcus marinus, wild-type and mutant A134F), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo Prochlorococcus marinus
4.1.99.5 additional information installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Synechococcus sp. RS9917), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo Synechococcus sp. RS9917
4.1.99.5 additional information installation of a recombinant hydrocarbon production system in Escherichia coli strain BL21(DE3)DELTAyqhDDELTAahr for production of n-alkanes by a combinant ion of four enzymes, i.e. aldehyde deformylating oxygenase (from Synechocystis sp. PCC 6803), ferredoxin (from Synechocystis), phosphopantetheinyl transferase (from Bacillus subtilis) and carboxylic acid reductase (from Mycobacterium marinum), method optimization and evaluation, overview. GC-MS analysis of the volatile alkanes produced. Comparison of ADO orthologues from different origins in hydrocarbon biosynthesis in vivo Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Nostoc punctiforme
-
an alkane + formate + H2O + 2 NADP+
-
?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Prochlorococcus marinus
-
an alkane + formate + H2O + 2 NADP+
-
?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Synechocystis sp. PCC 6803
-
an alkane + formate + H2O + 2 NADP+
-
?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Synechococcus sp. RS9917
-
an alkane + formate + H2O + 2 NADP+
-
?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Nostoc punctiforme ATCC 29133 / PCC 73102
-
an alkane + formate + H2O + 2 NADP+
-
?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Prochlorococcus marinus MIT 9313
-
an alkane + formate + H2O + 2 NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.5 Nostoc punctiforme B2J1M1
-
-
4.1.99.5 Nostoc punctiforme ATCC 29133 / PCC 73102 B2J1M1
-
-
4.1.99.5 Prochlorococcus marinus Q7V6D4
-
-
4.1.99.5 Prochlorococcus marinus MIT 9313 Q7V6D4
-
-
4.1.99.5 Synechococcus sp. RS9917 A3Z5H6
-
-
4.1.99.5 Synechocystis sp. PCC 6803 Q55688
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme an alkane + formate + H2O + 2 NADP+
-
?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus an alkane + formate + H2O + 2 NADP+
-
?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Synechocystis sp. PCC 6803 an alkane + formate + H2O + 2 NADP+
-
?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Synechococcus sp. RS9917 an alkane + formate + H2O + 2 NADP+
-
?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme ATCC 29133 / PCC 73102 an alkane + formate + H2O + 2 NADP+
-
?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus MIT 9313 an alkane + formate + H2O + 2 NADP+
-
?
4.1.99.5 additional information GC-MS analysis of the volatile alkane products Nostoc punctiforme ?
-
?
4.1.99.5 additional information GC-MS analysis of the volatile alkane products Prochlorococcus marinus ?
-
?
4.1.99.5 additional information GC-MS analysis of the volatile alkane products Synechocystis sp. PCC 6803 ?
-
?
4.1.99.5 additional information GC-MS analysis of the volatile alkane products Synechococcus sp. RS9917 ?
-
?
4.1.99.5 additional information GC-MS analysis of the volatile alkane products Nostoc punctiforme ATCC 29133 / PCC 73102 ?
-
?
4.1.99.5 additional information GC-MS analysis of the volatile alkane products Prochlorococcus marinus MIT 9313 ?
-
?
4.1.99.5 n-butanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme n-propane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-butanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus n-propane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-butanal + O2 + 2 NADPH + 2 H+
-
Synechocystis sp. PCC 6803 n-propane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-butanal + O2 + 2 NADPH + 2 H+
-
Synechococcus sp. RS9917 n-propane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-butanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme ATCC 29133 / PCC 73102 n-propane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-butanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus MIT 9313 n-propane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-hexanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme n-pentane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-hexanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus n-pentane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-hexanal + O2 + 2 NADPH + 2 H+
-
Synechocystis sp. PCC 6803 n-pentane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-hexanal + O2 + 2 NADPH + 2 H+
-
Synechococcus sp. RS9917 n-pentane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-hexanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme ATCC 29133 / PCC 73102 n-pentane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-hexanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus MIT 9313 n-pentane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-octanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme n-heptane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-octanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus n-heptane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-octanal + O2 + 2 NADPH + 2 H+
-
Synechocystis sp. PCC 6803 n-heptane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-octanal + O2 + 2 NADPH + 2 H+
-
Synechococcus sp. RS9917 n-heptane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-octanal + O2 + 2 NADPH + 2 H+
-
Nostoc punctiforme ATCC 29133 / PCC 73102 n-heptane + formate + H2O + 2 NADP+
-
?
4.1.99.5 n-octanal + O2 + 2 NADPH + 2 H+
-
Prochlorococcus marinus MIT 9313 n-heptane + formate + H2O + 2 NADP+
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.99.5 ADO
-
Nostoc punctiforme
4.1.99.5 ADO
-
Prochlorococcus marinus
4.1.99.5 ADO
-
Synechocystis sp. PCC 6803
4.1.99.5 ADO
-
Synechococcus sp. RS9917
4.1.99.5 aldehyde deformylating oxygenase
-
Nostoc punctiforme
4.1.99.5 aldehyde deformylating oxygenase
-
Prochlorococcus marinus
4.1.99.5 aldehyde deformylating oxygenase
-
Synechocystis sp. PCC 6803
4.1.99.5 aldehyde deformylating oxygenase
-
Synechococcus sp. RS9917
4.1.99.5 cyanobacterial aldehyde deformylating oxygenase
-
Nostoc punctiforme
4.1.99.5 cyanobacterial aldehyde deformylating oxygenase
-
Prochlorococcus marinus
4.1.99.5 cyanobacterial aldehyde deformylating oxygenase
-
Synechocystis sp. PCC 6803
4.1.99.5 cyanobacterial aldehyde deformylating oxygenase
-
Synechococcus sp. RS9917
4.1.99.5 PMT_1231
-
Prochlorococcus marinus
4.1.99.5 RS9917_09941
-
Synechococcus sp. RS9917

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.5 NADPH
-
Nostoc punctiforme
4.1.99.5 NADPH
-
Prochlorococcus marinus
4.1.99.5 NADPH
-
Synechocystis sp. PCC 6803
4.1.99.5 NADPH
-
Synechococcus sp. RS9917