EC Number | Application | Comment | Organism |
---|---|---|---|
4.1.2.9 | biofuel production | overexpression of the PktB isoform leads to a 2fold increase in intracellular acetyl-CoA concentration, and a 2.6fold yield enhancement from methane to microbial biomass and lipids compared to wild-type, increasing the potential for methanotroph lipid-based fuel production | Methylotuvimicrobium buryatense |
4.1.2.9 | synthesis | given that acetyl-CoA is a key intermediate in several biosynthetic pathways, phosphoketolase overexpression offers a viable strategy to enhance the economics of an array of biological methane conversion processes | Methylotuvimicrobium buryatense |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.9 | - |
Methylotuvimicrobium buryatense |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.9 | D-Xylulose 5-phosphate + phosphate | Methylotuvimicrobium buryatense | the phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. This pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO2 lost through pyruvate decarboxylation in the Embden-Meyerhof-Parnas pathway | Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O | - |
? | |
4.1.2.9 | D-Xylulose 5-phosphate + phosphate | Methylotuvimicrobium buryatense 5GB1S | the phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. This pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO2 lost through pyruvate decarboxylation in the Embden-Meyerhof-Parnas pathway | Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.9 | Methylotuvimicrobium buryatense | - |
- |
- |
4.1.2.9 | Methylotuvimicrobium buryatense 5GB1S | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.1.2.9 | culture condition:methane-grown cell | - |
Methylotuvimicrobium buryatense | - |
4.1.2.9 | culture condition:methanol-grown cell | - |
Methylotuvimicrobium buryatense | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.9 | D-Xylulose 5-phosphate + phosphate | - |
Methylotuvimicrobium buryatense | Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O | - |
? | |
4.1.2.9 | D-Xylulose 5-phosphate + phosphate | the phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. This pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO2 lost through pyruvate decarboxylation in the Embden-Meyerhof-Parnas pathway | Methylotuvimicrobium buryatense | Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O | - |
? | |
4.1.2.9 | D-Xylulose 5-phosphate + phosphate | - |
Methylotuvimicrobium buryatense 5GB1S | Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O | - |
? | |
4.1.2.9 | D-Xylulose 5-phosphate + phosphate | the phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. This pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO2 lost through pyruvate decarboxylation in the Embden-Meyerhof-Parnas pathway | Methylotuvimicrobium buryatense 5GB1S | Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O | - |
? |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.2.9 | metabolism | the phosphoketolase pathway functions in heterofermentative bacteria where carbon flux through two sugar catabolic pathways to mixed acids (lactic acid and acetic acid) increases cellular ATP production. This pathway also serves as an alternative route to produce acetyl-CoA that bypasses the CO2 lost through pyruvate decarboxylation in the Embden-Meyerhof-Parnas pathway | Methylotuvimicrobium buryatense |