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Literature summary extracted from

  • Sonntag, F.; Kroner, C.; Lubuta, P.; Peyraud, R.; Horst, A.; Buchhaupt, M.; Schrader, J.
    Engineering Methylobacterium extorquens for de novo synthesis of the sesquiterpenoid alpha-humulene from methanol (2015), Metab. Eng., 32, 82-94 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.104 gene zssI, recombinant coexpression of alpha-humulene synthase of Zingiber zerumbet from codon-optimized gene zssI with farnesyl diphosphate (FPP) synthase from Saccharomyces cerevisiae leads to concentrations of up to 18 mg/l alpha-humulene in Methylobacterium extorquens strain AM1, cloning scheme comprising genes zssI and ERG20 Zingiber zerumbet

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.104 additional information potential of Methylobacterium extorquens as a future platform host for the production of high-value terpenoids from the alternative carbon source methanol. Inducible recombinant coexpression of alpha-humulene synthasefrom Zingiber zerumbet with farnesyl diphosphate (FPP) synthase from Saccharomyces cerevisiae leads to concentrations of up to 18 mg/l alpha-humulene in Methylobacterium extorquens strain AM1. Extracellular alpha-humulene has no growth inhibiting effect on Methylobacterium extorquens strain AM1 up to concentrations of 1 g/l. Method evaluation, overview Zingiber zerumbet

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.104 (2E,6E)-farnesyl diphosphate Zingiber zerumbet
-
alpha-humulene + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.104 Zingiber zerumbet B1B1U3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.104 (2E,6E)-farnesyl diphosphate
-
Zingiber zerumbet alpha-humulene + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.104 zssI
-
Zingiber zerumbet