EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.2.5 | expression in Escherichia coli | Arthrobacter sp. MAT3885 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.5 | additional information | - |
chondroitin 6-sulfate | Km value of native enzyme is 0.058 mg/ml, of recombinant enzyme is 0.062 mg/ml, pH 7.0, 40°C | Arthrobacter sp. MAT3885 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.2.5 | 80000 | - |
gel filtration | Arthrobacter sp. MAT3885 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.5 | Arthrobacter sp. MAT3885 | I6QML9 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.2.5 | 8.5 | - |
substrate chondroitin 6-sulfate, pH 7.0, 40°C | Arthrobacter sp. MAT3885 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.5 | chondroitin 4-sulfate | 61% of the activity with hyaluronic acid | Arthrobacter sp. MAT3885 | ? | - |
? | |
4.2.2.5 | chondroitin 6-sulfate | 86% of the activity with hyaluronic acid | Arthrobacter sp. MAT3885 | ? | - |
? | |
4.2.2.5 | hyaluronic acid | - |
Arthrobacter sp. MAT3885 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.5 | monomer | 1 * 83456, calculated from sequence | Arthrobacter sp. MAT3885 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.5 | 40 | - |
- |
Arthrobacter sp. MAT3885 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.5 | 30 | - |
stable for 60 min | Arthrobacter sp. MAT3885 |
4.2.2.5 | 40 | - |
10 min, 65% residual activity for native enzyme, 90% residual activity for recombinant protein | Arthrobacter sp. MAT3885 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.5 | 6 | 7.5 | - |
Arthrobacter sp. MAT3885 |