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Literature summary extracted from

  • Zhang, W.; Zhang, T.; Jiang, B.; Mu, W.
    Biochemical characterization of a D-psicose 3-epimerase from Treponema primitia ZAS-1 and its application on enzymatic production of D-psicose (2016), J. Sci. Food. Agric., 96, 49-56 .
    View publication on PubMed

Application

EC Number Application Comment Organism
5.1.3.30 synthesis Trpr-DPEase might be appropriate for the industrial production of D-psicose. Comparison of D-psicose productivity of various ketose 3-epimerases, overview Treponema primitia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.30 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant overexpression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Treponema primitia

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.30 Ba2+ inactivation at 1 mM Treponema primitia
5.1.3.30 Ca2+ inactivation at 1 mM Treponema primitia
5.1.3.30 Cu2+ inactivation at 1 mM Treponema primitia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.30 additional information
-
additional information Michaelis-Menten kinetics Treponema primitia
5.1.3.30 0.209
-
D-psicose pH 8.0, 70°C, recombinant enzyme Treponema primitia
5.1.3.30 0.279
-
D-fructose pH 8.0, 70°C, recombinant enzyme Treponema primitia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.30 Co2+ highly activating at 1 mM, essential for the activity of the recombinant enzyme Treponema primitia
5.1.3.30 Mn2+ highly activating at 1 mM Treponema primitia
5.1.3.30 additional information the enzyme is metal-dependent Treponema primitia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.30 D-psicose Treponema primitia
-
D-fructose
-
r
5.1.3.30 D-psicose Treponema primitia ZAS-1
-
D-fructose
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.30 Treponema primitia
-
-
-
5.1.3.30 Treponema primitia ZAS-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.30 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Treponema primitia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.30 227.5
-
purified recombinant His6-tagged enzyme, pH 8.0, 70°C Treponema primitia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.30 D-fructose
-
Treponema primitia D-psicose
-
r
5.1.3.30 D-fructose
-
Treponema primitia ZAS-1 D-psicose
-
r
5.1.3.30 D-psicose
-
Treponema primitia D-fructose
-
r
5.1.3.30 D-psicose
-
Treponema primitia ZAS-1 D-fructose
-
r
5.1.3.30 additional information the D-psicose/D-fructose equilibrium ratio of Trpr-DPEase is 28:72. Recombinant enzyme produces D-psicose at a yield of 137.5 g/l from 500 g/l D-fructose Treponema primitia ?
-
?
5.1.3.30 additional information the D-psicose/D-fructose equilibrium ratio of Trpr-DPEase is 28:72. Recombinant enzyme produces D-psicose at a yield of 137.5 g/l from 500 g/l D-fructose Treponema primitia ZAS-1 ?
-
?

Subunits

EC Number Subunits Comment Organism
5.1.3.30 ? x * 33000, recombinant enzyme, SDS-PAGE Treponema primitia

Synonyms

EC Number Synonyms Comment Organism
5.1.3.30 Trpr-DPEase
-
Treponema primitia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.30 70
-
recombinant enzyme Treponema primitia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.30 40
-
purified recombinant His6-tagged enzyme, over 75% activity remaining after 4 h Treponema primitia
5.1.3.30 50
-
purified recombinant His6-tagged enzyme, 7% activity remaining after 4 h Treponema primitia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.30 292.88
-
D-fructose pH 8.0, 70°C, recombinant enzyme Treponema primitia
5.1.3.30 502.77
-
D-psicose pH 8.0, 70°C, recombinant enzyme Treponema primitia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.30 8
-
recombinant enzyme Treponema primitia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.30 6 9 over 80% of maximal activity within this range Treponema primitia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.3.30 6 9 purified recombinant enzyme, 4°C, 2 h, completely stable Treponema primitia

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.30 1049.8
-
D-fructose pH 8.0, 70°C, recombinant enzyme Treponema primitia
5.1.3.30 2405.6
-
D-psicose pH 8.0, 70°C, recombinant enzyme Treponema primitia