Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ma, L.; Ma, Q.; Cai, R.; Zong, Z.; Du, L.; Guo, G.; Zhang, Y.; Xiao, D.
    Effect of beta-mannanase domain from Trichoderma reesei on its biochemical characters and synergistic hydrolysis of sugarcane bagasse (2018), J. Sci. Food Agric., 98, 2540-2547 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 the full-length gene (Man1) and two truncated genes (Man1DELTACBM lacking CBM domain and Man1DELTALCBM lacking linker and CBM domains) from Trichoderma reesei Rut C30 are cloned and expressed in Pichia pastoris Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Trichoderma reesei Q99036
-
-
3.2.1.78 Trichoderma reesei RUT C-30 Q99036
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78
-
 Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 locust bean gum + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Trichoderma reesei RUT C-30 ?
-
 ?

Expression

EC Number Organism Comment Expression
3.2.1.78 Trichoderma reesei two truncated genes (Man1DELTACBM lacking carbohydrate-binding module domain and Man1DELTALCBM lacking linker and CBM domains) from Trichoderma reesei Rut C30 are cloned and expressed in Pichia pastoris. The expression efficiency is improved after truncating carbohydrate-binding module. Activities of Man1 and Man1DELTACBM in the culture supernatant after 168 h of induction are 34.5 and 42.9 U/ml. A value of 0.36 U/ml is detected for Man1DELTALCBM (lacking carbohydrate-binding module and linker). Man1 shows higher thermostability than Man1DELTACBM at low temperature, whereas Man1DELTACBM has a higher specificity for galactomannan (Km: 2.5 mg/ml) than Man1 (Km: 4.0 mg/mL) additional information

General Information

EC Number General Information Comment Organism
3.2.1.78 metabolism key enzyme for hydrolyzing mannan, a major constituent of hemicellulose Trichoderma reesei