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Literature summary extracted from

  • Li, Y.; Miao, M.; Chen, X.; Jiang, B.; Liu, M.; Feng, B.
    Improving the catalytic behavior of inulin fructotransferase under high hydrostatic pressure (2015), J. Sci. Food Agric., 95, 2588-2594 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.18 synthesis use of high hydrostatic pressure to improve the catalytic efficiency. The maximum activity of IFTase is obtained under 200 MPa at 60°C. High hydrostatic pressure lowers the energy barrier of the enzymatic reaction and decreases the volume between the reactants and the transition state. Under this condition, the optimal pH for the enzymatic reaction shifts from 5.5 to 6.0. The activity is further enhanced by 65.2% in the presence of 1.5 M NaCl Paenarthrobacter aurescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.18 0.442
-
 inulin pH 5.5, 60°C, pressure of 200 MPa Paenarthrobacter aurescens
4.2.2.18 0.493
-
 inulin pH 5.5, 60°C, pressure of 150 MPa Paenarthrobacter aurescens
4.2.2.18 0.538
-
 inulin pH 5.5, 60°C, pressure of 100 MPa Paenarthrobacter aurescens
4.2.2.18 0.57
-
 inulin pH 5.5, 60°C, pressure of 0.1 MPa Paenarthrobacter aurescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.18 Na+ the activity is enhanced by 65.2% in the presence of 1.5 M NaCl Paenarthrobacter aurescens

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.18 Paenarthrobacter aurescens F8QV43
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.18 inulin
-
 Paenarthrobacter aurescens di-D-fructofuranose 1,2':2,3' dianhydride + ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.18 1054
-
 inulin pH 5.5, 60°C, pressure of 0.1 MPa Paenarthrobacter aurescens
4.2.2.18 1244
-
 inulin pH 5.5, 60°C, pressure of 100 MPa Paenarthrobacter aurescens
4.2.2.18 1327
-
 inulin pH 5.5, 60°C, pressure of 150 MPa Paenarthrobacter aurescens
4.2.2.18 1437
-
 inulin pH 5.5, 60°C, pressure of 200 MPa Paenarthrobacter aurescens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.2.18 1860
-
 inulin pH 5.5, 60°C, pressure of 0.1 MPa Paenarthrobacter aurescens
4.2.2.18 2309
-
 inulin pH 5.5, 60°C, pressure of 100 MPa Paenarthrobacter aurescens
4.2.2.18 2691
-
 inulin pH 5.5, 60°C, pressure of 150 MPa Paenarthrobacter aurescens
4.2.2.18 3246
-
 inulin pH 5.5, 60°C, pressure of 200 MPa Paenarthrobacter aurescens