BRENDA - Enzyme Database

Expression and properties of the glyoxysomal and cytosolic forms of isocitrate lyase in Amaranthus caudatus L

Eprintsev, A.T.; Fedorin, D.N.; Salnikov, A.V.; Igamberdiev, A.U.; J. Plant Physiol. 181, 1-8 (2015)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Cu2+
isoforms ICL1 and ICL2 are 60-80% inhibited by 5 mM Cu2+
Amaranthus caudatus
4.1.3.1
Fe2+
isoforms ICL1 and ICL2 are 30% inhibited by 5 mM Fe2+
Amaranthus caudatus
4.1.3.1
Mg2+
isoform ICL2 is 20% inhibited by 5 mM Mg2+
Amaranthus caudatus
4.1.3.1
Mn2+
isoform ICL1 is 40% inhibited by 5 mM Mn2+
Amaranthus caudatus
4.1.3.1
Zn2+
isoforms ICL1 and ICL2 are 60-80% inhibited by 5 mM Zn2+
Amaranthus caudatus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.045
-
DL-isocitrate
isoform ICL1, with 5 mM MgCl2, at pH 7.5 and 25C
Amaranthus caudatus
4.1.3.1
0.063
-
DL-isocitrate
isoform ICL2, with 5 mM MgCl2, at pH 7.5 and 25C
Amaranthus caudatus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.3.1
cytosol
isoform ICL2
Amaranthus caudatus
5829
-
4.1.3.1
glyoxysome
isoform ICL1
Amaranthus caudatus
9514
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
isoform ICL1 is activated 3fold by 5 mM Mg2+
Amaranthus caudatus
4.1.3.1
Mn2+
isoform ICL2 is activated 3fold by 5 mM Mn2+
Amaranthus caudatus
4.1.3.1
additional information
isoform ICL2 is not activated by Mg2+. Ca2+and K+ reveal no marked effect on both isoforms of the enzyme
Amaranthus caudatus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Amaranthus caudatus
-
succinate + glyoxylate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Amaranthus caudatus
-
cultivar Kinelskiy 254
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-25 gel filtration
Amaranthus caudatus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
seedling
-
Amaranthus caudatus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.3.1
0.024
-
isoform ICL2, at pH 6.5 and 25C
Amaranthus caudatus
4.1.3.1
0.03
-
isoform ICL1, with 5 mM MnCl2, at pH 6.5 and 25C
Amaranthus caudatus
4.1.3.1
0.035
-
isoform ICL2, with 5 mM MnCl2, at pH 6.5 and 25C
Amaranthus caudatus
4.1.3.1
0.48
-
isoform ICL1, at pH 6.5 and 25C
Amaranthus caudatus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
DL-isocitrate
-
748480
Amaranthus caudatus
succinate + glyoxylate
-
-
-
r
4.1.3.1
isocitrate
-
748480
Amaranthus caudatus
succinate + glyoxylate
-
-
-
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.5
-
isoform ICL2
Amaranthus caudatus
4.1.3.1
7.5
-
isoform ICL1
Amaranthus caudatus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
Cu2+
isoforms ICL1 and ICL2 are 60-80% inhibited by 5 mM Cu2+
Amaranthus caudatus
4.1.3.1
Fe2+
isoforms ICL1 and ICL2 are 30% inhibited by 5 mM Fe2+
Amaranthus caudatus
4.1.3.1
Mg2+
isoform ICL2 is 20% inhibited by 5 mM Mg2+
Amaranthus caudatus
4.1.3.1
Mn2+
isoform ICL1 is 40% inhibited by 5 mM Mn2+
Amaranthus caudatus
4.1.3.1
Zn2+
isoforms ICL1 and ICL2 are 60-80% inhibited by 5 mM Zn2+
Amaranthus caudatus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.045
-
DL-isocitrate
isoform ICL1, with 5 mM MgCl2, at pH 7.5 and 25C
Amaranthus caudatus
4.1.3.1
0.063
-
DL-isocitrate
isoform ICL2, with 5 mM MgCl2, at pH 7.5 and 25C
Amaranthus caudatus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.3.1
cytosol
isoform ICL2
Amaranthus caudatus
5829
-
4.1.3.1
glyoxysome
isoform ICL1
Amaranthus caudatus
9514
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Mg2+
isoform ICL1 is activated 3fold by 5 mM Mg2+
Amaranthus caudatus
4.1.3.1
Mn2+
isoform ICL2 is activated 3fold by 5 mM Mn2+
Amaranthus caudatus
4.1.3.1
additional information
isoform ICL2 is not activated by Mg2+. Ca2+and K+ reveal no marked effect on both isoforms of the enzyme
Amaranthus caudatus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Amaranthus caudatus
-
succinate + glyoxylate
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-25 gel filtration
Amaranthus caudatus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
seedling
-
Amaranthus caudatus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.3.1
0.024
-
isoform ICL2, at pH 6.5 and 25C
Amaranthus caudatus
4.1.3.1
0.03
-
isoform ICL1, with 5 mM MnCl2, at pH 6.5 and 25C
Amaranthus caudatus
4.1.3.1
0.035
-
isoform ICL2, with 5 mM MnCl2, at pH 6.5 and 25C
Amaranthus caudatus
4.1.3.1
0.48
-
isoform ICL1, at pH 6.5 and 25C
Amaranthus caudatus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
DL-isocitrate
-
748480
Amaranthus caudatus
succinate + glyoxylate
-
-
-
r
4.1.3.1
isocitrate
-
748480
Amaranthus caudatus
succinate + glyoxylate
-
-
-
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.5
-
isoform ICL2
Amaranthus caudatus
4.1.3.1
7.5
-
isoform ICL1
Amaranthus caudatus
Expression
EC Number
Organism
Commentary
Expression
4.1.3.1
Amaranthus caudatus
the highest isoform ICL1 activity is observed on the 3rd day of germination; then the activity and expression of the corresponding gene decreases, while the activity of isoform ICL2 and gene expression increases to the 7th day of germination and then remains at the same level
up
General Information
EC Number
General Information
Commentary
Organism
4.1.3.1
metabolism
the function of isoform ICL1 is related to the glyoxylate cycle while isoform ICL2 functions independently from the glyoxylate cycle and interconverts organic acids in the cytosol
Amaranthus caudatus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.1.3.1
metabolism
the function of isoform ICL1 is related to the glyoxylate cycle while isoform ICL2 functions independently from the glyoxylate cycle and interconverts organic acids in the cytosol
Amaranthus caudatus
Expression (protein specific)
EC Number
Organism
Commentary
Expression
4.1.3.1
Amaranthus caudatus
the highest isoform ICL1 activity is observed on the 3rd day of germination; then the activity and expression of the corresponding gene decreases, while the activity of isoform ICL2 and gene expression increases to the 7th day of germination and then remains at the same level
up