EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.93 | gene AcC2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli | Aquilaria crassna |
4.2.3.104 | gene HS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli | Aquilaria crassna |
4.2.3.104 | gene HS2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli | Aquilaria crassna |
4.2.3.104 | gene HS3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, heterologous expression of the enzyme in Escherichia coli | Aquilaria crassna |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.3.93 | additional information | the enzyme has no N-terminal transit peptide | Aquilaria crassna | - |
- |
4.2.3.104 | additional information | the enzyme has no N-terminal transit peptide | Aquilaria crassna | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.93 | (2E,6E)-farnesyl diphosphate | Aquilaria crassna | - |
alpha-guaiene + diphosphate | - |
? | |
4.2.3.93 | (2E,6E)-farnesyl diphosphate | Aquilaria crassna | - |
delta-guaiene + diphosphate | - |
? | |
4.2.3.93 | additional information | Aquilaria crassna | the enzyme catalyzes the production of three sesquiterpenes, alpha-guaiene, alpha-humulene, and delta-guaiene | ? | - |
? | |
4.2.3.104 | (2E,6E)-farnesyl diphosphate | Aquilaria crassna | - |
alpha-humulene + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.93 | Aquilaria crassna | D0VMR6 | - |
- |
4.2.3.104 | Aquilaria crassna | A0A142F308 | - |
- |
4.2.3.104 | Aquilaria crassna | A0A142F309 | - |
- |
4.2.3.104 | Aquilaria crassna | A0A142F310 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
4.2.3.93 | additional information | the enzyme has no N-terminal transit peptide | Aquilaria crassna |
4.2.3.104 | additional information | the enzyme has no N-terminal transit peptide | Aquilaria crassna |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.3.93 | cell suspension culture | - |
Aquilaria crassna | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.93 | (2E,6E)-farnesyl diphosphate | - |
Aquilaria crassna | alpha-guaiene + diphosphate | - |
? | |
4.2.3.93 | (2E,6E)-farnesyl diphosphate | - |
Aquilaria crassna | delta-guaiene + diphosphate | - |
? | |
4.2.3.93 | additional information | the enzyme catalyzes the production of three sesquiterpenes, alpha-guaiene, alpha-humulene, and delta-guaiene | Aquilaria crassna | ? | - |
? | |
4.2.3.93 | additional information | solid-phase microextraction gas chromatography/mass spectrometry (SPME-GC/MS) analysis of the enzyme reaction products | Aquilaria crassna | ? | - |
? | |
4.2.3.104 | (2E,6E)-farnesyl diphosphate | - |
Aquilaria crassna | alpha-humulene + diphosphate | - |
? | |
4.2.3.104 | additional information | the recombinant alpha-humulene synthase isozyme HS1 produces 94.6% alpha-humulene and 5.4% beta-caryophyllene | Aquilaria crassna | ? | - |
? | |
4.2.3.104 | additional information | the recombinant alpha-humulene synthase isozyme HS2 produces 95.0% alpha-humulene and 5.0% beta-caryophyllene | Aquilaria crassna | ? | - |
? | |
4.2.3.104 | additional information | the recombinant alpha-humulene synthase isozyme HS3 produces 93.5% alpha-humulene and 6.5% beta-caryophyllene | Aquilaria crassna | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.93 | ACC2 | - |
Aquilaria crassna |
4.2.3.93 | delta-guaiene synthase 1 | - |
Aquilaria crassna |
4.2.3.104 | Hs1 | - |
Aquilaria crassna |
4.2.3.104 | Hs2 | - |
Aquilaria crassna |
4.2.3.104 | HS3 | - |
Aquilaria crassna |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.2.3.93 | Aquilaria crassna | methyl jasmonate induces the expression of enzyme delta-guaiene synthase, at 6 h after methyl jasmonate treatment, the expression of the delta-guaiene synthase gene has increased approximately 150fold resulting in 240 pmol/mg protein/h delta-guaiene production | up |
4.2.3.104 | Aquilaria crassna | methyl jasmonate slightly induces the expression of enzyme alpha-humulene synthase, at 6 h after methyl jasmonate treatment, the expression of the alpha-humulene synthase gene has increased approximately 1.5fold resulting in 330 pmol/mg protein/h alpha-humulene production | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.3.93 | metabolism | the enzyme is involved in the biosynthetic pathways for sesquiterpenes found in agarwood and cell suspension cultures, putative pathway, overview. Various post-transcriptional, translational, or post-translational regulatory mechanisms affecting the expression or activity of alpha-humulene and delta-guaiene synthases are possible | Aquilaria crassna |
4.2.3.93 | additional information | the enzyme contains conserved RPx8W motif and DDxxD motif, which is a divalent metal-ion substrate-binding site | Aquilaria crassna |
4.2.3.93 | physiological function | the enzyme is involved in the biosynthesis of the three sesquiterpenes, alpha-guaiene (cf. EC 4.2.3.87), alpha-humulene (cf. EC 4.2.3.104), and delta-guaiene, only very small amounts of alpha-humulene are produced | Aquilaria crassna |
4.2.3.104 | metabolism | the enzyme is involved in the biosynthetic pathways for sesquiterpenes found in agarwood and cell suspension cultures, putative pathway, overview. Various post-transcriptional, translational, or post-translational regulatory mechanisms affecting the expression or activity of alpha-humulene and delta-guaiene synthases are possible | Aquilaria crassna |
4.2.3.104 | additional information | the enzyme contains conserved RPx8W motif and DDxxD motif, which is a divalent metal-ion substrate-binding site | Aquilaria crassna |