EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.20 | expression in Escherichia coli BL21(DE3) | Thermoplasma acidophilum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.20 | acarbose | competitive, pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | Aesculin | mixed type of noncompetitive inhibition, pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | CuCl2 | 1 mM, 65% loss of activity | Thermoplasma acidophilum | |
3.2.1.20 | EDTA | 1 mM, 7% loss of activity | Thermoplasma acidophilum | |
3.2.1.20 | HgCl2 | 1 mM, 96% loss of activity | Thermoplasma acidophilum | |
3.2.1.20 | N-bromosuccinimide | 1 mM, 92% loss of activity | Thermoplasma acidophilum | |
3.2.1.20 | NiCl2 | 1 mM, 16% loss of activity | Thermoplasma acidophilum | |
3.2.1.20 | p-chloromercuribenzoate | 1 mM, 5% loss of activity | Thermoplasma acidophilum | |
3.2.1.20 | SDS | 1 mM, complete inactivation | Thermoplasma acidophilum | |
3.2.1.20 | ZnCl2 | 1 mM, 72% loss of activity | Thermoplasma acidophilum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | 0.47 | - |
maltotriose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 0.65 | - |
maltose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 0.67 | - |
maltotetraose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 1.24 | - |
kojibiose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 1.77 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 4.25 | - |
isomaltose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 6.6 | - |
panose | pH 5.5, 80°C | Thermoplasma acidophilum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.20 | intracellular | - |
Thermoplasma acidophilum | 5622 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.20 | MnCl2 | 1 mM, 1.6fold activation | Thermoplasma acidophilum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 409000 | - |
gel filtration | Thermoplasma acidophilum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.20 | Thermoplasma acidophilum | Q9HLD0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.20 | - |
Thermoplasma acidophilum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Thermoplasma acidophilum | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
3.2.1.20 | isomaltose + H2O | hydrolyzed to e lesser extent than maltose | Thermoplasma acidophilum | 2 D-glucose | - |
? | |
3.2.1.20 | kojibiose + H2O | hydrolyzed to e lesser extent than maltose | Thermoplasma acidophilum | 2 D-glucose | - |
? | |
3.2.1.20 | maltooligosaccharide + H2O | the enzyme shows highest specificity for maltose and decreasing values of catalytic efficiency (kcat/Km) toward higher maltooligosaccahrides. AglA specifically liberates glucose from the non-reducing end | Thermoplasma acidophilum | ? | - |
? | |
3.2.1.20 | maltopentaose + H2O | - |
Thermoplasma acidophilum | D-glucose + ? | - |
? | |
3.2.1.20 | maltose + H2O | - |
Thermoplasma acidophilum | 2 D-glucose | - |
? | |
3.2.1.20 | maltotetraose + H2O | - |
Thermoplasma acidophilum | D-glucose + ? | - |
? | |
3.2.1.20 | maltotriose + H2O | - |
Thermoplasma acidophilum | D-glucose + ? | - |
? | |
3.2.1.20 | additional information | structural analyses of two arbutin transglucosylation products of the transglucosylation reaction with arbutin and maltose indicates that the glucose unit of maltose was transferred to the C-3 and C-6 position in the glucose moiety of arbutin | Thermoplasma acidophilum | ? | - |
? | |
3.2.1.20 | nigerose + H2O | hydrolyzed to e lesser extent than maltose | Thermoplasma acidophilum | 2 D-glucose | - |
? | |
3.2.1.20 | panose + H2O | - |
Thermoplasma acidophilum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.20 | pentamer | 5 * 82000, SDS-PAGE | Thermoplasma acidophilum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.20 | AglA | - |
Thermoplasma acidophilum |
3.2.1.20 | Ta0298 | - |
Thermoplasma acidophilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 80 | - |
- |
Thermoplasma acidophilum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 60 | 90 | 60°C: about 55% of maximal activity, 90°C: about 75% of maximal activity | Thermoplasma acidophilum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 80 | - |
16.8 h, enzyme retains its full activity | Thermoplasma acidophilum |
3.2.1.20 | 85 | - |
half-life: 1.4 h | Thermoplasma acidophilum |
3.2.1.20 | 90 | - |
half-life: 12 min | Thermoplasma acidophilum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | 22.9 | - |
isomaltose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 35.9 | - |
panose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 59.7 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 85.5 | - |
maltotetraose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 90.2 | - |
maltotriose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 127 | - |
kojibiose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 143 | - |
maltose | pH 5.5, 80°C | Thermoplasma acidophilum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 5.5 | - |
substrate: 4-nitrophenyl alpha-D-glucopyranoside | Thermoplasma acidophilum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 4 | 7 | pH 4.0: about 55% of maximal activity, pH 7.0: about 70% of maximal activity | Thermoplasma acidophilum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | 0.00299 | - |
acarbose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 0.0043 | - |
Aesculin | pH 5.5, 80°C | Thermoplasma acidophilum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.20 | Thermoplasma acidophilum | calculated from sequence | - |
5.29 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | 5.4 | - |
isomaltose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 5.4 | - |
panose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 33.7 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 103 | - |
kojibiose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 128 | - |
maltotetraose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 194 | - |
maltotriose | pH 5.5, 80°C | Thermoplasma acidophilum | |
3.2.1.20 | 220 | - |
maltose | pH 5.5, 80°C | Thermoplasma acidophilum |