EC Number | Application | Comment | Organism |
---|---|---|---|
5.1.3.11 | food industry | the enzyme is of interest for the dairy industry due to their ability to convert lactose into epilactose and lactulose, rare disaccharides with prebiotic properties | Caldicellulosiruptor saccharolyticus |
5.1.3.11 | food industry | the enzyme is of interest for the dairy industry due to their ability to convert lactose into epilactose and lactulose, rare disaccharides with prebiotic properties | Dictyoglomus turgidum |
5.1.3.11 | pharmacology | synthesis of lactulose, which is used as a pharmaceutical against various illnesses, such as chronic constipation | Caldicellulosiruptor saccharolyticus |
5.1.3.11 | pharmacology | synthesis of lactulose, which is used as a pharmaceutical against various illnesses, such as chronic constipation | Dictyoglomus turgidum |
5.1.3.11 | synthesis | the enzyme from Caldicellulosiruptor saccharolyticus is applied for epilactose and lactulose production in milk at lower temperatures | Caldicellulosiruptor saccharolyticus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.3.11 | expression in Escherichia coli XL-1 | Caldicellulosiruptor saccharolyticus |
5.1.3.11 | expression in Escherichia coli XL-1 | Dictyoglomus turgidum |
5.1.3.11 | recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Caldicellulosiruptor saccharolyticus |
5.1.3.11 | recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Dictyoglomus turgidum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.3.11 | additional information | - |
additional information | Michaelis-Menten kinetics | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | additional information | - |
additional information | Michaelis-Menten kinetics | Dictyoglomus turgidum | |
5.1.3.11 | 257 | - |
lactulose | pH 7.5, 50°C | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 257 | - |
lactulose | pH 7.5, 50°C, recombinant enzyme | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 276 | - |
epilactose | pH 7.5, 50°C | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 276 | - |
epilactose | pH 7.5, 50°C, recombinant enzyme | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 339 | - |
lactulose | pH 7.5, 50°C | Dictyoglomus turgidum | |
5.1.3.11 | 339 | - |
lactulose | pH 7.5, 50°C, recombinant enzyme | Dictyoglomus turgidum | |
5.1.3.11 | 381 | - |
epilactose | pH 7.5, 50°C | Dictyoglomus turgidum | |
5.1.3.11 | 381 | - |
epilactose | pH 7.5, 50°C, recombinant enzyme | Dictyoglomus turgidum | |
5.1.3.11 | 462 | - |
lactose | pH 7.5, 50°C | Dictyoglomus turgidum | |
5.1.3.11 | 462 | - |
lactose | pH 7.5, 50°C, recombinant enzyme | Dictyoglomus turgidum | |
5.1.3.11 | 553 | - |
lactose | pH 7.5, 50°C | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 553 | - |
lactose | pH 7.5, 50°C, recombinant enzyme | Caldicellulosiruptor saccharolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 42000 | - |
- |
Caldicellulosiruptor saccharolyticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.3.11 | 2 lactulose | Caldicellulosiruptor saccharolyticus | - |
lactose + epilactose | - |
r | |
5.1.3.11 | 2 lactulose | Dictyoglomus turgidum | - |
lactose + epilactose | - |
r | |
5.1.3.11 | 2 lactulose | Dictyoglomus turgidum DSM 6724 | - |
lactose + epilactose | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.3.11 | Caldicellulosiruptor saccharolyticus | A4XGA6 | - |
- |
5.1.3.11 | Dictyoglomus turgidum | B8DZK4 | - |
- |
5.1.3.11 | Dictyoglomus turgidum DSM 6724 | B8DZK4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.3.11 | - |
Caldicellulosiruptor saccharolyticus |
5.1.3.11 | - |
Dictyoglomus turgidum |
5.1.3.11 | recombinant His-tagged enzyme 151fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by HRV 3C protease, desalting gel filtration, and another step of nickel affinity chromatography | Dictyoglomus turgidum |
5.1.3.11 | recombinant His-tagged enzyme 6.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by HRV 3C protease, desalting gel filtration, and another step of nickel affinity chromatography | Caldicellulosiruptor saccharolyticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 74.39 | - |
purified recombinant enzyme, pH 7.5, 50°C, substrate lactose | Caldicellulosiruptor saccharolyticus |
5.1.3.11 | 90.58 | - |
purified recombinant enzyme, pH 7.5, 50°C, substrate lactose | Dictyoglomus turgidum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.3.11 | 2 lactulose | - |
Caldicellulosiruptor saccharolyticus | lactose + epilactose | - |
r | |
5.1.3.11 | 2 lactulose | - |
Dictyoglomus turgidum | lactose + epilactose | - |
r | |
5.1.3.11 | 2 lactulose | - |
Dictyoglomus turgidum DSM 6724 | lactose + epilactose | - |
r | |
5.1.3.11 | epilactose | lactose production occurs earlier and faster than lactulose production. The lactose content decreases after an initial increase. Simultaneously, the epilactose content decreases rapidly during this first part of the conversion to about 26%. Therefore, 20 min into the conversion of epilactose as a starting substrate, an inversion of the amounts of the two sugars occurr, resulting in about 26% epilactose and 66% lactose | Caldicellulosiruptor saccharolyticus | lactose | - |
r | |
5.1.3.11 | epilactose | lactose production occurs earlier and faster than lactulose production. The lactose content decreases after an initial increase. Simultaneously,the epilactose content decreases rapidly during this first part of the conversion to about 26%. Therefore, 20 min into the conversion of epilactose as a starting substrate, an inversion of the amounts of the two sugars occurrs, resulting in about 26% epilactose and 66% lactose | Dictyoglomus turgidum | lactose | - |
r | |
5.1.3.11 | epilactose | lactose production occurs earlier and faster than lactulose production. The lactose content decreases after an initial increase. Simultaneously,the epilactose content decreases rapidly during this first part of the conversion to about 26%. Therefore, 20 min into the conversion of epilactose as a starting substrate, an inversion of the amounts of the two sugars occurrs, resulting in about 26% epilactose and 66% lactose | Dictyoglomus turgidum DSM 6724 | lactose | - |
r | |
5.1.3.11 | epilactose | lactose production occurs earlier and faster than lactulose production. The lactose content decreases after an initial increase. Simultaneously,the epilactose content decreases rapidly during this first part of the conversion to about 26%. Therefore, 20 min into the conversion of epilactose as a starting substrate, an inversion of the amounts of the two sugars occurr, resulting in about 26% epilactose and 66% lactose | Caldicellulosiruptor saccharolyticus | lactulose | - |
r | |
5.1.3.11 | epilactose | lactose production occurs earlier and faster than lactulose production. The lactose content decreases after an initial increase. Simultaneously,the epilactose content decreases rapidly during this first part of the conversion to about 26%. Therefore, 20 min into the conversion of epilactose as a starting substrate, an inversion of the amounts of the two sugars occurrs, resulting in about 26% epilactose and 66% lactose | Dictyoglomus turgidum | lactulose | - |
r | |
5.1.3.11 | epilactose | lactose production occurs earlier and faster than lactulose production. The lactose content decreases after an initial increase. Simultaneously,the epilactose content decreases rapidly during this first part of the conversion to about 26%. Therefore, 20 min into the conversion of epilactose as a starting substrate, an inversion of the amounts of the two sugars occurrs, resulting in about 26% epilactose and 66% lactose | Dictyoglomus turgidum DSM 6724 | lactulose | - |
r | |
5.1.3.11 | lactose | the epimerisation and, therefore, the production of epilactose can be seen to occure arlier and faster than the production of lactulose. After reaching a maximal ratio of about 28% after 10 min, the epilactose content decreases and so does the lactose content, while the lactulose content increases over the course of the whole conversion. The conversion reaches an apparent steady state with apparent final sugar ratios of 54.4% lactulose, 14.8% epilactose and 30.8% lactose | Dictyoglomus turgidum | epilactose | - |
r | |
5.1.3.11 | lactose | the epimerisation and, therefore, the production of epilactose can be seen to occure arlier and faster than the production of lactulose. After reaching a maximal ratio of about 28% after 10 min, the epilactose content decreases and so does the lactose content, while the lactulose content increases over the course of the whole conversion. The conversion reaches an apparent steady state with apparent final sugar ratios of 55.0% lactulose, 14.6% epilactose and 30.4% lactose | Caldicellulosiruptor saccharolyticus | epilactose | - |
r | |
5.1.3.11 | lactose | the epimerisation and, therefore, the production of epilactose can be seen to occure arlier and faster than the production of lactulose. After reaching a maximal ratio of about 28% after 10 min, the epilactose content decreases and so does the lactose content, while the lactulose content increases over the course of the whole conversion. The conversion reaches an apparent steady state with apparent final sugar ratios of 54.4% lactulose, 14.8% epilactose and 30.8% lactose | Dictyoglomus turgidum DSM 6724 | epilactose | - |
r | |
5.1.3.11 | lactose | the epimerisation and, therefore, the production of epilactose can be seen to occure arlier and faster than the production of lactulose. After reaching a maximal ratio of about 28% after 10 min, the epilactose content decreases and so does the lactose content, while the lactulose content increases over the course of the whole conversion. The conversion reaches an apparent steady state with apparent final sugar ratios of 54.4% lactulose, 14.8% epilactose and 30.8% lactose | Dictyoglomus turgidum | lactulose | - |
r | |
5.1.3.11 | lactose | the epimerisation and, therefore, the production of epilactose can be seen to occure earlier and faster than the production of lactulose. After reaching a maximal ratio of about 28% after 10 min, the epilactose content decreases and so does the lactose content, while the lactulose content increases over the course of the whole conversion. The conversion reaches an apparent steady state with apparent final sugar ratios of 55.0% lactulose, 14.6% epilactose and 30.4% lactose | Caldicellulosiruptor saccharolyticus | lactulose | - |
r | |
5.1.3.11 | lactose | the epimerisation and, therefore, the production of epilactose can be seen to occure arlier and faster than the production of lactulose. After reaching a maximal ratio of about 28% after 10 min, the epilactose content decreases and so does the lactose content, while the lactulose content increases over the course of the whole conversion. The conversion reaches an apparent steady state with apparent final sugar ratios of 54.4% lactulose, 14.8% epilactose and 30.8% lactose | Dictyoglomus turgidum DSM 6724 | lactulose | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.1.3.11 | ? | x * 42000, recombinant detagged enzyme, SDS-PAGE, x * 47300, about sequence calculation | Caldicellulosiruptor saccharolyticus |
5.1.3.11 | ? | x * 42000, recombinant detagged enzyme, SDS-PAGE, x * 47500, about sequence calculation | Dictyoglomus turgidum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.3.11 | cellobiose 2-epimerase | - |
Caldicellulosiruptor saccharolyticus |
5.1.3.11 | cellobiose 2-epimerase | - |
Dictyoglomus turgidum |
5.1.3.11 | CsCE | - |
Caldicellulosiruptor saccharolyticus |
5.1.3.11 | DsCE | - |
Dictyoglomus turgidum |
5.1.3.11 | DtCE | - |
Dictyoglomus turgidum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 50 | - |
- |
Caldicellulosiruptor saccharolyticus |
5.1.3.11 | 50 | - |
- |
Dictyoglomus turgidum |
5.1.3.11 | 70 | - |
- |
Dictyoglomus turgidum |
5.1.3.11 | 80 | - |
- |
Caldicellulosiruptor saccharolyticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.3.11 | 0.54 | - |
lactulose | pH 7.5, 50°C | Dictyoglomus turgidum | |
5.1.3.11 | 0.54 | - |
lactulose | pH 7.5, 50°C, recombinant enzyme | Dictyoglomus turgidum | |
5.1.3.11 | 0.88 | - |
lactulose | pH 7.5, 50°C | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 0.88 | - |
lactulose | pH 7.5, 50°C, recombinant enzyme | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 101 | - |
epilactose | pH 7.5, 50°C | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 101 | - |
epilactose | pH 7.5, 50°C, recombinant enzyme | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 125 | - |
epilactose | pH 7.5, 50°C | Dictyoglomus turgidum | |
5.1.3.11 | 125 | - |
epilactose | pH 7.5, 50°C, recombinant enzyme | Dictyoglomus turgidum | |
5.1.3.11 | 237 | - |
lactose | pH 7.5, 50°C | Dictyoglomus turgidum | |
5.1.3.11 | 237 | - |
lactose | pH 7.5, 50°C, recombinant enzyme | Dictyoglomus turgidum | |
5.1.3.11 | 303 | - |
lactose | pH 7.5, 50°C | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 303 | - |
lactose | pH 7.5, 50°C, recombinant enzyme | Caldicellulosiruptor saccharolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 7 | - |
- |
Dictyoglomus turgidum |
5.1.3.11 | 7.5 | - |
- |
Caldicellulosiruptor saccharolyticus |
5.1.3.11 | 7.5 | - |
- |
Dictyoglomus turgidum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.3.11 | 0.0016 | - |
lactulose | pH 7.5, 50°C | Dictyoglomus turgidum | |
5.1.3.11 | 0.0016 | - |
lactulose | pH 7.5, 50°C, recombinant enzyme | Dictyoglomus turgidum | |
5.1.3.11 | 0.0034 | - |
lactulose | pH 7.5, 50°C | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 0.0034 | - |
lactulose | pH 7.5, 50°C, recombinant enzyme | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 0.33 | - |
epilactose | pH 7.5, 50°C | Dictyoglomus turgidum | |
5.1.3.11 | 0.33 | - |
epilactose | pH 7.5, 50°C, recombinant enzyme | Dictyoglomus turgidum | |
5.1.3.11 | 0.37 | - |
epilactose | pH 7.5, 50°C | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 0.37 | - |
epilactose | pH 7.5, 50°C, recombinant enzyme | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 0.51 | - |
lactose | pH 7.5, 50°C | Dictyoglomus turgidum | |
5.1.3.11 | 0.51 | - |
lactose | pH 7.5, 50°C, recombinant enzyme | Dictyoglomus turgidum | |
5.1.3.11 | 0.55 | - |
lactose | pH 7.5, 50°C | Caldicellulosiruptor saccharolyticus | |
5.1.3.11 | 0.55 | - |
lactose | pH 7.5, 50°C, recombinant enzyme | Caldicellulosiruptor saccharolyticus |