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Literature summary extracted from

  • Zhang, W.; Li, H.; Zhang, T.; Jiang, B.; Zhou, L.; Mu, W.
    Characterization of a D-psicose 3-epimerase from Dorea sp. CAG317 with an acidic pH optimum and a high specific activity (2015), J. Mol. Catal. B, 120, 68-74 .
No PubMed abstract available

Application

EC Number Application Comment Organism
5.1.3.30 synthesis the recombinant Dorea sp. DPEase displays significantly higher specific activity at acidic pHs and remarkably higher productivity of D-psicose at pH 6.0, indicating that it is appropriate for use as a different source of D-psicose producing enzyme Dorea sp. CAG:317

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.30 gene dpe, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Dorea sp. CAG:317

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.30 Ba2+
-
 Dorea sp. CAG:317
5.1.3.30 Ca2+
-
 Dorea sp. CAG:317
5.1.3.30 Cu2+
-
 Dorea sp. CAG:317

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.30 Co2+ best metal cofactor, has no effect on pH stability and slightly imporves temperature Dorea sp. CAG:317
5.1.3.30 Fe2+ 26.59% of the activity with Co2+ at 1 mM Dorea sp. CAG:317
5.1.3.30 Mn2+ 81.24% of the activity with Co2+ at 1 mM Dorea sp. CAG:317
5.1.3.30 additional information metal-dependent enzyme, no activity without presence of divalent metal ion Dorea sp. CAG:317
5.1.3.30 Ni2+ 7.96% of the activity with Co2+ at 1 mM Dorea sp. CAG:317
5.1.3.30 Zn2+ 1.75% of the activity with Co2+ at 1 mM Dorea sp. CAG:317

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.30 D-psicose Dorea sp. CAG:317
-
 D-fructose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.30 Dorea sp. CAG:317
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.30 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Dorea sp. CAG:317

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.30 806
-
 purified recombinant enzyme, pH 6.0, 70°C, 1 mM Co2+ Dorea sp. CAG:317

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.30 D-fructose
-
 Dorea sp. CAG:317 D-psicose
-
 r
5.1.3.30 D-psicose
-
 Dorea sp. CAG:317 D-fructose
-
 r
5.1.3.30 additional information the equilibrium ratio between D-fructose and D-psicose is approximately 30:70 for the recombinant enzyme, elevated temperature does not significantly shift the equilibrium toward D-psicose. The conversion ratio of D-fructose to D-psicose is calculated to be 30.1%, 31.5%, 31.8%, 31.4%, 31.1%, 31.0%, 30.4%, 29.2%, and 28.6% when the reaction temperature is 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, and 80°C, respectively Dorea sp. CAG:317 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.3.30 homotetramer 4 * 33000, SDS-PAGE Dorea sp. CAG:317

Synonyms

EC Number Synonyms Comment Organism
5.1.3.30 DPEase
-
 Dorea sp. CAG:317

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.30 70
-
-
 Dorea sp. CAG:317

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.30 additional information
-
 Co2+ slightly improves the thermostability of the enzyme Dorea sp. CAG:317
5.1.3.30 40 70 the purified recombinant enzyme retains 70% and 30% of its initial activity after 4 h of incubation at 40°C and 50°C, respectively, 55% activity remaining after 0.5 h at 60°C, complete inactivation after 0.5 h at 70°C Dorea sp. CAG:317

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.30 6
-
-
 Dorea sp. CAG:317

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.3.30 5.5 8.5 the purified recombinant enzyme retains more than 80% of initial activity when incubated for 2 h at pH values from pH 5.5 to pH 8.5 Dorea sp. CAG:317

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.30 3.32
-
 D-fructose recombinant enzyme, pH 6.0, 70°C Dorea sp. CAG:317
5.1.3.30 6.9
-
 D-psicose recombinant enzyme, pH 6.0, 70°C Dorea sp. CAG:317