Literature summary extracted from
Todea, A.; Hiseni, A.; Otten, L.; Arends, I.; Peter, F.; Boeriu, C.
Increase of stability of oleate hydratase by appropriate immobilization technique and conditions (2015), J. Mol. Catal. B, 119, 40-47 .
No PubMed abstract available
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.2.1.53 |
expression in Escherichia coli |
Elizabethkingia meningoseptica |
General Stability
EC Number |
General Stability |
Organism |
---|
4.2.1.53 |
immobilization results in a radical improvement of operational stability of the enzyme, as the covalently bound enzyme preserves 75% of the initial activity after five reuses |
Elizabethkingia meningoseptica |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.1.53 |
Elizabethkingia meningoseptica |
C7DLJ6 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.1.53 |
- |
Elizabethkingia meningoseptica |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
4.2.1.53 |
4°C, 7 days, the native enzyme loses 60% of its activity |
Elizabethkingia meningoseptica |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.1.53 |
oleate + H2O |
- |
Elizabethkingia meningoseptica |
(R)-10-hydroxystearate |
- |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
4.2.1.53 |
30 |
- |
assay at |
Elizabethkingia meningoseptica |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
4.2.1.53 |
50 |
- |
the immobilized enzyme (covalent immobilization of the enzyme using glutaraldehyde as cross-linking agent with chitosan-magnetic composite as carrier) preserves 40% of the initial activity, while the native enzyme is completely inactivated |
Elizabethkingia meningoseptica |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.2.1.53 |
8 |
- |
assay at |
Elizabethkingia meningoseptica |