EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.3.11 | gene Tsac_2329, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Thermoanaerobacterium saccharolyticum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.3.11 | Co2+ | 35% inhibition at 1 mM | Thermoanaerobacterium saccharolyticum | |
5.1.3.11 | Cu2+ | 20% inhibition at 1 mM | Thermoanaerobacterium saccharolyticum | |
5.1.3.11 | additional information | no inhibition by EDTA | Thermoanaerobacterium saccharolyticum | |
5.1.3.11 | Zn2+ | 20% inhibition at 1 mM | Thermoanaerobacterium saccharolyticum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.3.11 | 124.7 | - |
lactose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum | |
5.1.3.11 | 241.1 | - |
cellobiose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.1.3.11 | additional information | the enzyme is metal independent | Thermoanaerobacterium saccharolyticum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.3.11 | lactose | Thermoanaerobacterium saccharolyticum | - |
epilactose | - |
r | |
5.1.3.11 | lactose | Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | - |
epilactose | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.3.11 | Thermoanaerobacterium saccharolyticum | I3VXT6 | - |
- |
5.1.3.11 | Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | I3VXT6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.3.11 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Thermoanaerobacterium saccharolyticum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 13.5 | - |
substrate lactose, recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum |
5.1.3.11 | 15.48 | - |
substrate cellobiose, recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum |
5.1.3.11 | 29.8 | - |
substrate mannobiose, recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.3.11 | 4-O-beta-D-glucosyl-D-mannose | - |
Thermoanaerobacterium saccharolyticum | cellobiose | - |
r | |
5.1.3.11 | beta-1,4-mannobiose | best substrate | Thermoanaerobacterium saccharolyticum | 4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
5.1.3.11 | beta-1,4-mannobiose | best substrate | Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | 4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
5.1.3.11 | cellobiose | - |
Thermoanaerobacterium saccharolyticum | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
5.1.3.11 | cellobiose | - |
Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
5.1.3.11 | lactose | - |
Thermoanaerobacterium saccharolyticum | epilactose | - |
r | |
5.1.3.11 | lactose | at pH 7.0 and 60°C, 50 mM epilactose is produced from 200 mM lactose by a 600 nM of enzyme concentration after reaction for 4 h | Thermoanaerobacterium saccharolyticum | epilactose | - |
r | |
5.1.3.11 | lactose | - |
Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | epilactose | - |
r | |
5.1.3.11 | lactose | at pH 7.0 and 60°C, 50 mM epilactose is produced from 200 mM lactose by a 600 nM of enzyme concentration after reaction for 4 h | Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | epilactose | - |
r | |
5.1.3.11 | additional information | no activity with N-acyl-D-glucosamine and D-glucose | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
5.1.3.11 | additional information | no activity with N-acyl-D-glucosamine and D-glucose | Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.1.3.11 | ? | x * 46500, recombinant enzyme, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.3.11 | epilactose-producing cellobiose 2-epimerase | - |
Thermoanaerobacterium saccharolyticum |
5.1.3.11 | Thsa-CE | - |
Thermoanaerobacterium saccharolyticum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 60 | - |
- |
Thermoanaerobacterium saccharolyticum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.3.11 | 30.9 | - |
lactose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum | |
5.1.3.11 | 100.4 | - |
cellobiose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 7 | - |
- |
Thermoanaerobacterium saccharolyticum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.3.11 | 0.248 | - |
lactose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum | |
5.1.3.11 | 0.417 | - |
cellobiose | recombinant enzyme, pH 7.0, 60°C | Thermoanaerobacterium saccharolyticum |