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Literature summary extracted from

  • Chen, Q.; Zhang, W.; Zhang, T.; Jiang, B.; Mu, W.
    Characterization of an epilactose-producing cellobiose 2-epimerase from Thermoanaerobacterium saccharolyticum (2015), J. Mol. Catal. B, 116, 39-44 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.11 gene Tsac_2329, phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Thermoanaerobacterium saccharolyticum

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.11 Co2+ 35% inhibition at 1 mM Thermoanaerobacterium saccharolyticum
5.1.3.11 Cu2+ 20% inhibition at 1 mM Thermoanaerobacterium saccharolyticum
5.1.3.11 additional information no inhibition by EDTA Thermoanaerobacterium saccharolyticum
5.1.3.11 Zn2+ 20% inhibition at 1 mM Thermoanaerobacterium saccharolyticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.11 124.7
-
lactose recombinant enzyme, pH 7.0, 60°C Thermoanaerobacterium saccharolyticum
5.1.3.11 241.1
-
cellobiose recombinant enzyme, pH 7.0, 60°C Thermoanaerobacterium saccharolyticum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.11 additional information the enzyme is metal independent Thermoanaerobacterium saccharolyticum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.11 lactose Thermoanaerobacterium saccharolyticum
-
epilactose
-
r
5.1.3.11 lactose Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691
-
epilactose
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.11 Thermoanaerobacterium saccharolyticum I3VXT6
-
-
5.1.3.11 Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 I3VXT6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.11 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Thermoanaerobacterium saccharolyticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.11 13.5
-
substrate lactose, recombinant enzyme, pH 7.0, 60°C Thermoanaerobacterium saccharolyticum
5.1.3.11 15.48
-
substrate cellobiose, recombinant enzyme, pH 7.0, 60°C Thermoanaerobacterium saccharolyticum
5.1.3.11 29.8
-
substrate mannobiose, recombinant enzyme, pH 7.0, 60°C Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.11 4-O-beta-D-glucosyl-D-mannose
-
Thermoanaerobacterium saccharolyticum cellobiose
-
r
5.1.3.11 beta-1,4-mannobiose best substrate Thermoanaerobacterium saccharolyticum 4-O-beta-D-mannopyranosyl-D-glucose
-
r
5.1.3.11 beta-1,4-mannobiose best substrate Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 4-O-beta-D-mannopyranosyl-D-glucose
-
r
5.1.3.11 cellobiose
-
Thermoanaerobacterium saccharolyticum 4-O-beta-D-glucopyranosyl-D-mannose
-
r
5.1.3.11 cellobiose
-
Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 4-O-beta-D-glucopyranosyl-D-mannose
-
r
5.1.3.11 lactose
-
Thermoanaerobacterium saccharolyticum epilactose
-
r
5.1.3.11 lactose at pH 7.0 and 60°C, 50 mM epilactose is produced from 200 mM lactose by a 600 nM of enzyme concentration after reaction for 4 h Thermoanaerobacterium saccharolyticum epilactose
-
r
5.1.3.11 lactose
-
Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 epilactose
-
r
5.1.3.11 lactose at pH 7.0 and 60°C, 50 mM epilactose is produced from 200 mM lactose by a 600 nM of enzyme concentration after reaction for 4 h Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 epilactose
-
r
5.1.3.11 additional information no activity with N-acyl-D-glucosamine and D-glucose Thermoanaerobacterium saccharolyticum ?
-
?
5.1.3.11 additional information no activity with N-acyl-D-glucosamine and D-glucose Thermoanaerobacterium saccharolyticum JW/SL-YS485 / DSM 8691 ?
-
?

Subunits

EC Number Subunits Comment Organism
5.1.3.11 ? x * 46500, recombinant enzyme, SDS-PAGE Thermoanaerobacterium saccharolyticum

Synonyms

EC Number Synonyms Comment Organism
5.1.3.11 epilactose-producing cellobiose 2-epimerase
-
Thermoanaerobacterium saccharolyticum
5.1.3.11 Thsa-CE
-
Thermoanaerobacterium saccharolyticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.11 60
-
-
Thermoanaerobacterium saccharolyticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.11 30.9
-
lactose recombinant enzyme, pH 7.0, 60°C Thermoanaerobacterium saccharolyticum
5.1.3.11 100.4
-
cellobiose recombinant enzyme, pH 7.0, 60°C Thermoanaerobacterium saccharolyticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.11 7
-
-
Thermoanaerobacterium saccharolyticum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.11 0.248
-
lactose recombinant enzyme, pH 7.0, 60°C Thermoanaerobacterium saccharolyticum
5.1.3.11 0.417
-
cellobiose recombinant enzyme, pH 7.0, 60°C Thermoanaerobacterium saccharolyticum