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Literature summary extracted from

  • Alagoz, D.; Tükel, S.; Yildirim, D.
    Purification, immobilization and characterization of (R)-hydroxynitrile lyase from Prunus amygdalus turcomanica seeds and their applicability for synthesis of enantiopure cyanohydrins (2014), J. Mol. Catal. B, 101, 40-46 .
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.1.2.10 the immobilized enzyme preparations (immobilized onto Eupergit CM and Eupergit C 250 L supports) are successfully reused 10times without loss of activity and enantioselectivity Prunus turcomanica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.10 100000
-
 HPLC Prunus turcomanica

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Prunus turcomanica
-
 Lincz.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10
-
 Prunus turcomanica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.10 seed
-
 Prunus turcomanica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 (R)-mandelonitrile
-
 Prunus turcomanica cyanide + benzaldehyde
-
 r
4.1.2.10 cyanide + 2-chlorobenzaldehyde
-
 Prunus turcomanica (R)-2-chloromandelonitrile
-
 r
4.1.2.10 cyanide + 3,4-dihydroxybenzaldehyde
-
 Prunus turcomanica (R)-3,4-dihydroxymandelonitrile
-
 r
4.1.2.10 cyanide + 3-phenylpropionaldehyde
-
 Prunus turcomanica (R)-2-hydroxy-4-phenyl butyronitrile
-
 r
4.1.2.10 cyanide + benzaldehyde
-
 Prunus turcomanica (R)-mandelonitrile
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.2.10 homotetramer 4 * 25000, SDS-PAGE Prunus turcomanica

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 (R)-hydroxynitrile lyase
-
 Prunus turcomanica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 25
-
 soluble enzyme and enzyme immobilized onto Eupergit CM and Eupergit C 250 L supports Prunus turcomanica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.10 22
-
 the relative activity of free enzyme decreases rapidly and depletes at the end of 15 days storage time while the immobilized hydroxynitrile lyases are very active at the same conditions with retained initial activities of 67% and 83% for hydroxynitrile lyase immobilized onto Eupergit CM and hydroxynitrile lyase immobilized onto Eupergit C 250 L, respectively. At the end of 30 days storage time, the corresponding retained activities are 48% and 71% for hydroxynitrile lyase immobilized onto Eupergit CM and hydroxynitrile lyase immobilized onto Eupergit C 250 L Prunus turcomanica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 5.5
-
 enzyme immobilized onto Eupergit CM and Eupergit C 250 L supports Prunus turcomanica
4.1.2.10 6
-
 soluble enzyme Prunus turcomanica