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Literature summary extracted from

  • Seo, P.W.; Ryu, H.C.; Gu, D.H.; Park, H.S.; Park, S.Y.; Kim, J.S.
    Structures of Zymomonas 2-keto-3-deoxy-6-phosphogluconate aldolase with and without a substrate analog at the phosphate-binding loop (2018), J. Microbiol. Biotechnol., 28, 1339-1345 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.14 expressed in Escherichia coli BL21 Star (DE3) cells Zymomonas mobilis subsp. mobilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.14 apoform and in complex with 3-phosphoglycerate, hanging drop vapor diffusion method, using 25% (w/v) polyethylene glycol 8,000 and 0.1 M Bis-Tris (pH 5.5) for the apoform or 1.6 M ammonium sulfate, 0 .1 M CAPS (pH 10.5), and 0.2 M lithium sulfate for the 3-phosphoglycerate-bound form Zymomonas mobilis subsp. mobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-gluconate Zymomonas mobilis subsp. mobilis
-
 pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-gluconate Zymomonas mobilis subsp. mobilis ATCC 31821
-
 pyruvate + D-glyceraldehyde 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.14 Zymomonas mobilis subsp. mobilis Q00384
-
-
4.1.2.14 Zymomonas mobilis subsp. mobilis ATCC 31821 Q00384
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.14 Ni-ion affinity chromatography and ion exchange column chromatography Zymomonas mobilis subsp. mobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 Zymomonas mobilis subsp. mobilis pyruvate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.14 2-dehydro-3-deoxy-6-phospho-D-gluconate
-
 Zymomonas mobilis subsp. mobilis ATCC 31821 pyruvate + D-glyceraldehyde 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.14 2-keto-3-deoxy-6-phosphogluconate aldolase
-
 Zymomonas mobilis subsp. mobilis
4.1.2.14 KDPG aldolase
-
 Zymomonas mobilis subsp. mobilis