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Literature summary extracted from

  • McLean, C.J.; Marles-Wright, J.; Custodio, R.; Lowther, J.; Kennedy, A.J.; Pollock, J.; Clarke, D.J.; Brown, A.R.; Campopiano, D.J.
    Characterization of homologous sphingosine-1-phosphate lyase isoforms in the bacterial pathogen Burkholderia pseudomallei (2017), J. Lipid Res., 58, 137-150 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.27 expressed in Escherichia coli BL21(DE3) cells Burkholderia pseudomallei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.27 hanging drop vapor diffusion method, using 20% (w/v) polypropylene glycol 400 and 10% (v/v) 1-propanol Burkholderia pseudomallei

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.27 K271A inactive Burkholderia pseudomallei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.27 0.0194
-
 (2E)-hexadecenal at pH 7.5 and 37°C Burkholderia pseudomallei
4.1.2.27 0.0482
-
 sphinganine 1-phosphate at pH 7.5 and 37°C Burkholderia pseudomallei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.27 sphinganine 1-phosphate Burkholderia pseudomallei
-
 phosphoethanolamine + palmitaldehyde
-
 ir
4.1.2.27 sphinganine 1-phosphate Burkholderia pseudomallei K96243
-
 phosphoethanolamine + palmitaldehyde
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.27 Burkholderia pseudomallei Q63IP8
-
-
4.1.2.27 Burkholderia pseudomallei K96243 Q63IP8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.27 Ni-NTA resin column chromatography and Superdex S-200 gel filtration Burkholderia pseudomallei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.27 (2E)-hexadecenal + semicarbazide
-
 Burkholderia pseudomallei (2E)-hexadecenal semicarbazone
-
 ?
4.1.2.27 (2E)-hexadecenal + semicarbazide
-
 Burkholderia pseudomallei K96243 (2E)-hexadecenal semicarbazone
-
 ?
4.1.2.27 D-erythrosphingosine 8% activity compared to sphinganine 1-phosphate Burkholderia pseudomallei ?
-
 ?
4.1.2.27 D-erythrosphingosine 8% activity compared to sphinganine 1-phosphate Burkholderia pseudomallei K96243 ?
-
 ?
4.1.2.27 sphinganine 1-phosphate
-
 Burkholderia pseudomallei phosphoethanolamine + palmitaldehyde
-
 ir
4.1.2.27 sphinganine 1-phosphate
-
 Burkholderia pseudomallei K96243 phosphoethanolamine + palmitaldehyde
-
 ir

Synonyms

EC Number Synonyms Comment Organism
4.1.2.27 S1P lyase
-
 Burkholderia pseudomallei
4.1.2.27 S1PL
-
 Burkholderia pseudomallei
4.1.2.27 S1PL2021
-
 Burkholderia pseudomallei
4.1.2.27 sphingosine-1-phosphate lyase
-
 Burkholderia pseudomallei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.27 0.015
-
 sphinganine 1-phosphate at pH 7.5 and 37°C Burkholderia pseudomallei
4.1.2.27 0.22
-
 (2E)-hexadecenal at pH 7.5 and 37°C Burkholderia pseudomallei

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.27 pyridoxal 5'-phosphate
-
 Burkholderia pseudomallei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.27 0.32
-
 sphinganine 1-phosphate at pH 7.5 and 37°C Burkholderia pseudomallei