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Literature summary extracted from

  • Martin del Campo, J.; Chun, Y.; Kim, J.; Patino, R.; Zhang, Y.
    Discovery and characterization of a novel ATP/polyphosphate xylulokinase from a hyperthermophilic bacterium Thermotoga maritima (2013), J. Ind. Microbiol. Biotechnol., 40, 661-669 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.17 expressed in Escherichia coli BL21 Star (DE3) Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.17 0.14
-
 ATP 37°C, pH 7.5 Thermotoga maritima
2.7.1.17 0.22
-
 D-xylulose 37°C, pH 7.5 Thermotoga maritima
2.7.1.17 0.7
-
 ATP 60°C, pH 7.5 Thermotoga maritima
2.7.1.17 1.24
-
 D-xylulose 60°C, pH 7.5 Thermotoga maritima
2.7.1.17 17
-
 (Polyphosphate)n 37°C, pH 7.5 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.17 60000
-
 SDS-PAGE Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.17 Thermotoga maritima Q9WXX1
-
-
2.7.1.17 Thermotoga maritima DSM 3109 Q9WXX1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.17 (polyphosphate)n + D-xylulose activity with polyphosphate is 41 % of its specific activity on ATP Thermotoga maritima (polyphosphate)n-1 + D-xylulose 5-phosphate
-
 ?
2.7.1.17 (polyphosphate)n + D-xylulose activity with polyphosphate is 41 % of its specific activity on ATP Thermotoga maritima DSM 3109 (polyphosphate)n-1 + D-xylulose 5-phosphate
-
 ?
2.7.1.17 ATP + D-xylulose
-
 Thermotoga maritima ADP + D-xylulose 5-phosphate
-
 ?
2.7.1.17 ATP + D-xylulose
-
 Thermotoga maritima DSM 3109 ADP + D-xylulose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.17 ? x * 60000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.7.1.17 ATP/polyphosphate xylulokinase
-
 Thermotoga maritima
2.7.1.17 TM0116
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.17 85
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.17 65 95 65°C: about 60% of maximal activity, 95°: about 60% of maximal activity Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.17 60
-
 degradation constants at 0.1 and 0.01 g/l xylulokinase at 60°C are 0.20/h and 0.35/h, respectively. The presence of 2 mM xylulose enhances the thermostability of 0.01 g/l xylulokinase, increasing degradation constants to 0.29/h Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.17 8.77
-
 (Polyphosphate)n 37°C, pH 7.5 Thermotoga maritima
2.7.1.17 24
-
 ATP 37°C, pH 7.5 Thermotoga maritima
2.7.1.17 26
-
 D-xylulose 37°C, pH 7.5 Thermotoga maritima
2.7.1.17 78
-
 D-xylulose 60°C, pH 7.5 Thermotoga maritima
2.7.1.17 83
-
 ATP 60°C, pH 7.5 Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.17 62
-
 D-xylulose 60°C, pH 7.5 Thermotoga maritima
2.7.1.17 116
-
 D-xylulose 37°C, pH 7.5 Thermotoga maritima
2.7.1.17 119
-
 ATP 60°C, pH 7.5 Thermotoga maritima
2.7.1.17 171
-
 ATP 37°C, pH 7.5 Thermotoga maritima
2.7.1.17 516
-
 (Polyphosphate)n 37°C, pH 7.5 Thermotoga maritima