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Literature summary extracted from

  • Siwinska, J.; Siatkowska, K.; Olry, A.; Grosjean, J.; Hehn, A.; Bourgaud, F.; Meharg, A.A.; Carey, M.; Lojkowska, E.; Ihnatowicz, A.
    Scopoletin 8-hydroxylase a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis (2018), J. Exp. Bot., 69, 1735-1748 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.60 expression of S8H protein in Nicotiana benthamiana leaves Arabidopsis thaliana
1.14.11.60 heterologous expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.60 Fe2+ concentrations above 0.05 mM significantly reduce the enzyme activity, leading to decreased fraxetin synthesis Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.60 0.011
-
 scopoletin pH 8.0, 31.5°C Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.60 Fe2+ enzyme efficiency is best in the presence of 0.05 mM Fe2+ Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.60 scopoletin + 2-oxoglutarate + O2 Arabidopsis thaliana the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis fraxetin + succinate + CO2
-
 ?
1.14.11.60 scopoletin + 2-oxoglutarate + O2 Arabidopsis thaliana Col-0 the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis fraxetin + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.60 Arabidopsis thaliana Q9LE86
-
-
1.14.11.60 Arabidopsis thaliana Col-0 Q9LE86
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.60
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.60 root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.60 scopoletin + 2-oxoglutarate + O2
-
 Arabidopsis thaliana fraxetin + succinate + CO2
-
 ?
1.14.11.60 scopoletin + 2-oxoglutarate + O2 the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis Arabidopsis thaliana fraxetin + succinate + CO2
-
 ?
1.14.11.60 scopoletin + 2-oxoglutarate + O2
-
 Arabidopsis thaliana Col-0 fraxetin + succinate + CO2
-
 ?
1.14.11.60 scopoletin + 2-oxoglutarate + O2 the enzyme is involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis Arabidopsis thaliana Col-0 fraxetin + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.60 At3g12900 protein
-
 Arabidopsis thaliana
1.14.11.60 S8H
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.60 31.5
-
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.11.60 1.73
-
 scopoletin pH 8.0, 31.5°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.60 8
-
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.14.11.60 metabolism the enzyme is involved in coumarin biosynthesis and is part of mechanisms used by plants to assimilate iron Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.11.60 157.3
-
 scopoletin pH 8.0, 31.5°C Arabidopsis thaliana