Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Durdagi, S.; Korkmaz, N.; Isik, S.; Vullo, D.; Astley, D.; Ekinci, D.; Salmas, R.E.; Senturk, M.; Supuran, C.T.
    Kinetic and docking studies of cytosolic/tumor-associated carbonic anhydrase isozymes I, II and IX with some hydroxylic compounds (2016), J. Enzyme Inhib. Med. Chem., 31, 1214-1220 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 recombinant expression of GST-tagged isozyme hCA IX in Escherichia coli strain BL21 Codon Plus Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 (2E)-2-[(4-hydroxy-3-methylphenyl)methylidene]-N-(5-sulfanylidene-4,5-dihydro-1,3,4-thiadiazol-2-yl)hydrazine-1-carboxamide
-
 Homo sapiens
4.2.1.1 (2E)-2-[(4-hydroxyphenyl)methylidene]-N-(5-sulfanylidene-4,5-dihydro-1,3,4-thiadiazol-2-yl)hydrazine-1-carboxamide
-
 Homo sapiens
4.2.1.1 2-coumaric acid
-
 Homo sapiens
4.2.1.1 acetazolamide i.e. 5-acetamido-1,3,4-thiadiazole-2-sulfonamide; i.e. 5-acetamido-1,3,4-thiadiazole-2-sulfonamide; i.e. 5-acetamido-1,3,4-thiadiazole-2-sulfonamide Homo sapiens
4.2.1.1 catechol
-
 Homo sapiens
4.2.1.1 curcumin
-
 Homo sapiens
4.2.1.1 dobutamin
-
 Homo sapiens
4.2.1.1 dobutamine
-
 Homo sapiens
4.2.1.1 additional information inhibitor synthesis and isozyme in silico docking study using enzyme structure, PDB ID 2FW4, modeling; inhibitor synthesis and isozyme in silico docking study using enzyme structure, PDB ID 3IAI, modeling. No inhibition by pyrogallol; inhibitor synthesis and isozyme in silico docking study using enzyme structure, PDB ID 5AML, modeling Homo sapiens
4.2.1.1 N-(1-(2-hydroxyphenylamino)-3-methyl-1-oxobutan-2-ylcarbamothioyl)benzamide i.e. NK-154, binding structure, overview; i.e. NK-154, binding structure, overview; i.e. NK-154, binding structure, overview Homo sapiens
4.2.1.1 N-(1-(2-hydroxyphenylamino)-4-methyl-1-oxopentan-2-ylcarbamothioyl) benzamide i.e. NK-168, binding structure, overview; i.e. NK-168, binding structure, overview; i.e. NK-168, binding structure, overview Homo sapiens
4.2.1.1 phenol
-
 Homo sapiens
4.2.1.1 propofol
-
 Homo sapiens
4.2.1.1 pyrogallol
-
 Homo sapiens
4.2.1.1 resorcinol
-
 Homo sapiens
4.2.1.1 resveratrol
-
 Homo sapiens
4.2.1.1 salicylic acid
-
 Homo sapiens
4.2.1.1 thioxolone
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ required, metalloenzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Homo sapiens
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Homo sapiens P00915
-
-
4.2.1.1 Homo sapiens P00918
-
-
4.2.1.1 Homo sapiens Q16790
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 native isozyme hCA I from erythrocytes isolated from human blood by cellulose-benzyl-sulfanilamide affinity chromatography, elution with 1 M NaCl/25 mM Na2HPO4, pH 6.3 Homo sapiens
4.2.1.1 native isozyme hCA II from erythrocytes isolated from human blood by cellulose-benzyl-sulfanilamide affinity chromatography, elution with 0.1 M CH3COONa/0.5 M NaClO4, pH 5.6 Homo sapiens
4.2.1.1 recombinant GST-tagged isozyme hCA IX from Escherichia coli strain BL21 Codon Plus by glutathione affinity chromatography and sulfonamide affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.1 erythrocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Homo sapiens CO2 + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 alpha-CA
-
 Homo sapiens
4.2.1.1 CA1
-
 Homo sapiens
4.2.1.1 CA2
-
 Homo sapiens
4.2.1.1 CA9
-
 Homo sapiens
4.2.1.1 carbonic anhydrase 1
-
 Homo sapiens
4.2.1.1 carbonic anhydrase 2
-
 Homo sapiens
4.2.1.1 carbonic anhydrase 9
-
 Homo sapiens
4.2.1.1 hCA I
-
 Homo sapiens
4.2.1.1 HCA II
-
 Homo sapiens
4.2.1.1 hCA IX
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.000012
-
 acetazolamide pH 8.3, 22°C Homo sapiens
4.2.1.1 0.000025
-
 acetazolamide pH 8.3, 22°C Homo sapiens
4.2.1.1 0.000068
-
 N-(1-(2-hydroxyphenylamino)-4-methyl-1-oxopentan-2-ylcarbamothioyl) benzamide pH 8.3, 22°C Homo sapiens
4.2.1.1 0.000074
-
 N-(1-(2-hydroxyphenylamino)-3-methyl-1-oxobutan-2-ylcarbamothioyl)benzamide pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00025
-
 acetazolamide pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00038
-
 curcumin pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00048
-
 dobutamin pH 8.3, 22°C Homo sapiens
4.2.1.1 0.000481
-
 N-(1-(2-hydroxyphenylamino)-3-methyl-1-oxobutan-2-ylcarbamothioyl)benzamide above, pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00049
-
 N-(1-(2-hydroxyphenylamino)-4-methyl-1-oxopentan-2-ylcarbamothioyl) benzamide above, pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00054
-
 pyrogallol pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00192
-
 dobutamin pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00241
-
 curcumin pH 8.3, 22°C Homo sapiens
4.2.1.1 0.0031
-
 2-coumaric acid pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00334
-
 (2E)-2-[(4-hydroxy-3-methylphenyl)methylidene]-N-(5-sulfanylidene-4,5-dihydro-1,3,4-thiadiazol-2-yl)hydrazine-1-carboxamide pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00383
-
 (2E)-2-[(4-hydroxyphenyl)methylidene]-N-(5-sulfanylidene-4,5-dihydro-1,3,4-thiadiazol-2-yl)hydrazine-1-carboxamide pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00405
-
 curcumin pH 8.3, 22°C Homo sapiens
4.2.1.1 0.0055
-
 phenol pH 8.3, 22°C Homo sapiens
4.2.1.1 0.0071
-
 salicylic acid pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00715
-
 (2E)-2-[(4-hydroxyphenyl)methylidene]-N-(5-sulfanylidene-4,5-dihydro-1,3,4-thiadiazol-2-yl)hydrazine-1-carboxamide pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00741
-
 pyrogallol pH 8.3, 22°C Homo sapiens
4.2.1.1 0.0077
-
 resorcinol pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00787
-
 salicylic acid pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00803
-
 (2E)-2-[(4-hydroxyphenyl)methylidene]-N-(5-sulfanylidene-4,5-dihydro-1,3,4-thiadiazol-2-yl)hydrazine-1-carboxamide pH 8.3, 22°C Homo sapiens
4.2.1.1 0.0088
-
 phenol pH 8.3, 22°C Homo sapiens
4.2.1.1 0.0092
-
 2-coumaric acid pH 8.3, 22°C Homo sapiens
4.2.1.1 0.00982
-
 dobutamine pH 8.3, 22°C Homo sapiens
4.2.1.1 0.0099
-
 catechol pH 8.3, 22°C Homo sapiens
4.2.1.1 0.0099
-
 salicylic acid pH 8.3, 22°C Homo sapiens
4.2.1.1 0.0102
-
 phenol pH 8.3, 22°C Homo sapiens
4.2.1.1 0.021
-
 (2E)-2-[(4-hydroxy-3-methylphenyl)methylidene]-N-(5-sulfanylidene-4,5-dihydro-1,3,4-thiadiazol-2-yl)hydrazine-1-carboxamide pH 8.3, 22°C Homo sapiens
4.2.1.1 0.0697
-
 resorcinol pH 8.3, 22°C Homo sapiens
4.2.1.1 0.115
-
 catechol pH 8.3, 22°C Homo sapiens
4.2.1.1 0.35
-
 (2E)-2-[(4-hydroxy-3-methylphenyl)methylidene]-N-(5-sulfanylidene-4,5-dihydro-1,3,4-thiadiazol-2-yl)hydrazine-1-carboxamide pH 8.3, 22°C Homo sapiens
4.2.1.1 0.795
-
 resorcinol pH 8.3, 22°C Homo sapiens
4.2.1.1 1
-
 2-coumaric acid above, pH 8.3, 22°C Homo sapiens
4.2.1.1 1
-
 N-(1-(2-hydroxyphenylamino)-3-methyl-1-oxobutan-2-ylcarbamothioyl)benzamide above, pH 8.3, 22°C Homo sapiens
4.2.1.1 1
-
 N-(1-(2-hydroxyphenylamino)-4-methyl-1-oxopentan-2-ylcarbamothioyl) benzamide above, pH 8.3, 22°C Homo sapiens
4.2.1.1 4.003
-
 catechol pH 8.3, 22°C Homo sapiens