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Literature summary extracted from
- D-Psicose 3-epimerase secretory overexpression, immobilization, and D-psicose biotransformation, separation and crystallization (2018), J. Chem. Technol. Biotechnol., 93, 350-357 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.3.30 | gene dpe, recombinant food-grade overexpression of His-tagged enzyme in Bacillus pumilus using the P2 promoter, derived from a cell wall protein promoter of the host bacterium, the recombinant protein is soluble and bioactive, and is secreted to a high leve, subcloning in Escherichis coli strain DH5alphal | Ruminococcus sp. 5_1_39BFAA |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.1.3.30 | additional information | immobilization of recombinant extracellular enzyme DPE onto anion exchange resin D301, DPE immobilization yield is 90%. D-Psicose is produced using the immobilized enzyme, separated by simulated moving bed chromatography (SMB), and purified by crystallization. The purity of the D-psicose crystals reaches 99.1%, method optimization, overview | Ruminococcus sp. 5_1_39BFAA |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.3.30 | Ca2+ | inhibits the recombinant immobilized enzyme | Ruminococcus sp. 5_1_39BFAA |  |
| 5.1.3.30 | Co2+ | inhibits the recombinant immobilized enzyme | Ruminococcus sp. 5_1_39BFAA | |
| 5.1.3.30 | Cu2+ | inhibits the recombinant immobilized enzyme | Ruminococcus sp. 5_1_39BFAA | |
| 5.1.3.30 | Fe3+ | inhibits the recombinant immobilized enzyme | Ruminococcus sp. 5_1_39BFAA | |
| 5.1.3.30 | Mg2+ | inhibits the recombinant immobilized enzyme | Ruminococcus sp. 5_1_39BFAA | |
| 5.1.3.30 | Ni2+ | inhibits the recombinant immobilized enzyme | Ruminococcus sp. 5_1_39BFAA | |
| 5.1.3.30 | Zn2+ | inhibits the recombinant immobilized enzyme | Ruminococcus sp. 5_1_39BFAA | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.3.30 | Mn2+ | activates the recombinant free and immobilized enzyme, but does not affect the reaction equilibrium for both the free and the immobilized enzyme | Ruminococcus sp. 5_1_39BFAA | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.30 | D-psicose | Ruminococcus sp. 5_1_39BFAA | - |
D-fructose | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.30 | Ruminococcus sp. 5_1_39BFAA | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.30 | 24.06 | - |
purified recombinant immobilized enzyme, pH 8.0. 60°C | Ruminococcus sp. 5_1_39BFAA |
| 5.1.3.30 | 58.6 | - |
recombinant free enzyme, pH 8.0. 60°C | Ruminococcus sp. 5_1_39BFAA |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.30 | D-fructose | 59.8% activity compared to the activity with D-psicose | Ruminococcus sp. 5_1_39BFAA | D-psicose | - |
r | |
| 5.1.3.30 | D-psicose | - |
Ruminococcus sp. 5_1_39BFAA | D-fructose | - |
r | |
| 5.1.3.30 | D-psicose | best substrate | Ruminococcus sp. 5_1_39BFAA | D-fructose | - |
r | |
| 5.1.3.30 | D-sorbose | 2.9% activity compared to the activity with D-psicose | Ruminococcus sp. 5_1_39BFAA | D-tagatose | - |
r | |
| 5.1.3.30 | D-tagatose | 24.2% activity compared to the activity with D-psicose | Ruminococcus sp. 5_1_39BFAA | D-sorbose | - |
r | |
| 5.1.3.30 | D-xylulose | 3.9% activity compared to the activity with D-psicose | Ruminococcus sp. 5_1_39BFAA | D-ribulose | - |
r | |
| 5.1.3.30 | additional information | substrate specificity, overview. Poor activity with D-galactose, D-glucose, and D-arabinose, no activity with D-mannose | Ruminococcus sp. 5_1_39BFAA | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.3.30 | DPE | - |
Ruminococcus sp. 5_1_39BFAA |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.30 | 60 | - |
free and immobilized recombinant enzyme | Ruminococcus sp. 5_1_39BFAA |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.30 | 20 | 60 | activity of free DPE has small decreases after being maintained at 20-40°C, and has moderate decreases at 50-60°C, while the thermal stability of the immobilized DPE increases, particularly at 50°C | Ruminococcus sp. 5_1_39BFAA |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.30 | 8 | - |
free recombinant enzyme | Ruminococcus sp. 5_1_39BFAA |
| 5.1.3.30 | 9 | - |
immobilized recombinant enzyme | Ruminococcus sp. 5_1_39BFAA |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.30 | 6 | 11 | immobilized recombinant enzyme | Ruminococcus sp. 5_1_39BFAA |
| 5.1.3.30 | 7 | 9 | free recombinant enzyme | Ruminococcus sp. 5_1_39BFAA |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 5.1.3.30 | Ruminococcus sp. 5_1_39BFAA | about, sequence calculation | - |
5 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.1.3.30 | evolution | the enzyme DPE from Ruminococcus sp. belongs to the D-tagatose 3-epimerase (DTE) family enzymes | Ruminococcus sp. 5_1_39BFAA |
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