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Literature summary extracted from

  • Rivero, C.W.; De Benedetti, E.C.; Gallego, F.L.; Pessela, B.C.; Guisan, J.M.; Trelles, J.A.
    Biosynthesis of an antiviral compound using a stabilized phosphopentomutase by multipoint covalent immobilization (2017), J. Biotechnol., 249, 34-41 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.7 alpha-D-Glucose 1,6-bisphosphate about 7fold increase of activity at 0.001 mM Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.7 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

General Stability

EC Number General Stability Organism
5.4.2.7 using 10% (v/v) glycerol, the enzyme maintains 86% of its initial activity at pH 10.0 after 18 h incubation Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.7 Mn2+ highest activity at 0.1 mM Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.7 alpha-D-ribose 1-phosphate Escherichia coli
-
 D-ribose 5-phosphate
-
 ?
5.4.2.7 alpha-D-ribose 1-phosphate Escherichia coli ATCC 4157
-
 D-ribose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.7 Escherichia coli
-
-
-
5.4.2.7 Escherichia coli ATCC 4157
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.7 HisTrap column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.7 alpha-D-ribose 1-phosphate
-
 Escherichia coli D-ribose 5-phosphate
-
 ?
5.4.2.7 alpha-D-ribose 1-phosphate
-
 Escherichia coli ATCC 4157 D-ribose 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.2.7 ? x * 43000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
5.4.2.7 PPM
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.2.7 50
-
 enzyme immobilized on CNBr-Ag is completely inactive after 16 h incubation at 50°C. Enzymes immobilized on cyanogen bromide or glyoxyl agarose maintain 100% of their initial activity after 16 h at 50°C. Enzyme immobilized on monoaminoethyl-N-aminoethyl-glutaraldehyde or monoaminoethyl-N-aminoethyl-agarose loses more than 50% of its initial activity after 16 h at 50°C Escherichia coli