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Literature summary extracted from

  • Liu, X.B.; Liu, M.; Tao, X.Y.; Zhang, Z.X.; Wang, F.Q.; Wei, D.Z.
    Metabolic engineering of Pichia pastoris for the production of dammarenediol-II (2015), J. Biotechnol., 216, 47-55 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.125 gene DDS, recombinant expression in Pichia pastoris using the promoter of the AOX1 gene, coexpression with an ERG1 gene and an ERG7 gene Panax ginseng

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.125 additional information metabolic engineering of Pichia pastoris strain GS115 for the high yield production of dammarenediol-II, based on the native triterpene synthetic pathway, a dammarenediol-II synthetic pathway is established in Pichia pastoris by introducing a dammarenediol-II synthase gene (PgDDS) from Panax ginseng. To increase the supply of 2,3-oxidosqualene, the ERG1 gene, which is responsible for 2,3-oxidosqualene synthesis, is coexpressed. And to reduce competition for 2,3-oxidosqualene from ergosterol biosynthesis without affecting the normal growth of Pichia pastoris, the ERG7gene, which is responsible for conversion of 2,3-oxidosqualene to lanosterol, is downregulated by replacing its native promoter with a thiamine-repressible promoter, using a marker-recycling and gene-targeting Cre- lox71/66 system, developed for Pichia pastoris. Method evaluation and optimization, overview Panax ginseng

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.125 (3S)-2,3-epoxy-2,3-dihydrosqualene + H2O Panax ginseng
-
dammarenediol II
-
r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.125 Panax ginseng Q08IT1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.125 (3S)-2,3-epoxy-2,3-dihydrosqualene + H2O
-
Panax ginseng dammarenediol II
-
r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.125 dammarenediol-II synthase
-
Panax ginseng
4.2.1.125 DDS
-
Panax ginseng
4.2.1.125 PgDDS
-
Panax ginseng
4.2.1.125 PNA
-
Panax ginseng

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.125 30
-
assay at Panax ginseng

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.125 7.4
-
assay at Panax ginseng

General Information

EC Number General Information Comment Organism
4.2.1.125 physiological function dammarenediol-II synthase gene (PgDDS) from Panax ginseng is responsible for the cyclization of 2,3-oxidosqualene to dammarenediol-II, the nucleus of dammarane-type ginsenosides, which are a group of active triterpenoids exhibiting various pharmacological activities Panax ginseng