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Literature summary extracted from

  • Li, M.; Liu, C.; Chen, H.; Deng, L.; Zhang, H.; Nian, R.; Xian, M.
    Biochemical characterization of isoprene synthase from Ipomoea batatas (2019), J. Biosci. Bioeng., 127, 138-144 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.27 expressed in Escherichia coli BL21(DE3) cells Ipomoea batatas

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.27 Ca2+ no activity in the presence of Ca2+ Ipomoea batatas
4.2.3.27 EDTA no activity in the presence of EDTA Ipomoea batatas

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.27 0.2
-
 dimethylallyl diphosphate at pH 7.8 and 37°C Ipomoea batatas
4.2.3.27 0.34
-
 dimethylallyl diphosphate at pH 7.8 and 37°C Populus alba

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.27 Mg2+ Mg2+ with optimum concentration of 56 mM is needed for enzyme activation Ipomoea batatas

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Populus alba
-
 isoprene + diphosphate
-
 ?
4.2.3.27 dimethylallyl diphosphate Ipomoea batatas
-
 isoprene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Ipomoea batatas
-
-
-
4.2.3.27 Populus alba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.27 BeyoGold His-tag purification resin column chromatography Ipomoea batatas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate
-
 Populus alba isoprene + diphosphate
-
 ?
4.2.3.27 dimethylallyl diphosphate
-
 Ipomoea batatas isoprene + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.27 ? x * 63000, SDS-PAGE Ipomoea batatas
4.2.3.27 ? x * 65000, SDS-PAGE Populus alba

Synonyms

EC Number Synonyms Comment Organism
4.2.3.27 ISPS
-
 Populus alba
4.2.3.27 ISPS
-
 Ipomoea batatas

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.27 42
-
-
 Ipomoea batatas

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.27 30 60 after incubation at 30°C for 10 min, 62% of the enzyme activity remains. 8% of the enzyme activity remains after 60 min of incubation at 30°C. After 10 min at 40°C, most of the enzyme activity (84%) is lost. Almost no enzyme activity remains after 40 min at 40°C. When the enzyme is incubated at 50°C or 60°C, no enzyme activity is detected after 10 min Ipomoea batatas

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.27 0.011
-
 dimethylallyl diphosphate at pH 7.8 and 37°C Populus alba
4.2.3.27 0.37
-
 dimethylallyl diphosphate at pH 7.8 and 37°C Ipomoea batatas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.27 8.6
-
-
 Ipomoea batatas

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.3.27 7
-
 89% enzyme activity is lost after 20 min of incubation at pH 7.0 Ipomoea batatas