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Literature summary extracted from

  • Mai Hoa, B.; Hibi, T.; Nasuno, R.; Matsuo, G.; Sasano, Y.; Takagi, H.
    Production of N-acetyl cis-4-hydroxy-l-proline by the yeast N-acetyltransferase Mpr1 (2012), J. Biosci. Bioeng., 114, 160-165 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.271 expressed in Escherichia coli JM109 cells Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.271 0.38
-
 cis-4-hydroxy-L-proline at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.62
-
 L-azetidine-2-carboxylate at pH 8.5 and 30°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.271 acetyl-CoA + cis-4-hydroxy-L-proline Saccharomyces cerevisiae
-
 CoA + N-acetyl-cis-4-hydroxy-L-proline
-
 ?
2.3.1.271 acetyl-CoA + cis-4-hydroxy-L-proline Saccharomyces cerevisiae Sigma1278b
-
 CoA + N-acetyl-cis-4-hydroxy-L-proline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.271 Saccharomyces cerevisiae E9P8D2
-
-
2.3.1.271 Saccharomyces cerevisiae Sigma1278b E9P8D2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.271 Ni-NTA His Bind resin column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.271 acetyl-CoA + cis-4-hydroxy-L-proline
-
 Saccharomyces cerevisiae CoA + N-acetyl-cis-4-hydroxy-L-proline
-
 ?
2.3.1.271 acetyl-CoA + cis-4-hydroxy-L-proline
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-cis-4-hydroxy-L-proline
-
 ?
2.3.1.271 acetyl-CoA + L-azetidine-2-carboxylate
-
 Saccharomyces cerevisiae CoA + N-acetyl-L-azetidine-2-carboxylate
-
 ?
2.3.1.271 acetyl-CoA + L-azetidine-2-carboxylate
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-L-azetidine-2-carboxylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.271 MPR1
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.271 5.19
-
 cis-4-hydroxy-L-proline at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 13.57
-
 L-azetidine-2-carboxylate at pH 8.5 and 30°C Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.271 8.38
-
 L-azetidine-2-carboxylate at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 13.66
-
 cis-4-hydroxy-L-proline at pH 8.5 and 30°C Saccharomyces cerevisiae