Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lange, C.; Kiesel, S.; Peters, S.; Virus, S.; Scheer, H.; Jahn, D.; Moser, J.
    Broadened substrate specificity of 3-hydroxyethyl bacteriochlorophyllide a dehydrogenase (BchC) indicates a new route for the biosynthesis of bacteriochlorophyll a (2015), J. Biol. Chem., 290, 19697-19709 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.165 gene bchF, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Chlorobaculum tepidum

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.165 additional information reconstitution of a bacteriochlorophyll formation pathway consisting of dark-operative protochlorophyllide oxidoreductase, BchF, and BchC, with chlorin 3-hydroxyethyl chlorophyllide as a natural substrate identified for BchC. Modification of the isocyclic E ring, omission of the central magnesium ion, zinc as an alternative metal ion, and a non-reduced B ring system are tolerated by BchC. According to this broadened in vitro activity, the chlorin 3-hydroxyethyl chlorophyllide a is identified as a natural substrate of BchC in a reconstituted pathway consisting of dark-operative protochlorophyllide oxidoreductase, BchF, and BchC. The established reaction sequence allows for an additional new branching point for the synthesis of bacteriochlorophyll a Chlorobaculum tepidum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.165 membrane integral transmembrane protein, the enzyme contains four putative membrane-spanning helices comprising the transmembrane segments Ile23-Tyr42, Thr56-Glu77, Phe94-Gln113, and His118-Gln140 Chlorobaculum tepidum 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.165 a 3-(1-hydroxyethyl) bacteriochlorophyllide a Chlorobaculum tepidum
-
 a 3-vinyl bacteriochlorophyllide a + H2O
-
 ?
4.2.1.165 a 3-(1-hydroxyethyl) bacteriochlorophyllide a Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS
-
 a 3-vinyl bacteriochlorophyllide a + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.165 Chlorobaculum tepidum Q8KBL0
-
-
4.2.1.165 Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS Q8KBL0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.165 the recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) is efficiently isolated by detergent solubilization and ultracentrifugation from the host membrane fraction Chlorobaculum tepidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.165 a 3-(1-hydroxyethyl) bacteriochlorophyllide a
-
 Chlorobaculum tepidum a 3-vinyl bacteriochlorophyllide a + H2O
-
 ?
4.2.1.165 a 3-(1-hydroxyethyl) bacteriochlorophyllide a
-
 Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS a 3-vinyl bacteriochlorophyllide a + H2O
-
 ?
4.2.1.165 additional information coupled enzymatic assays of BchF and BchC are initiated in an anoxic chamber, using either crude cellular BchF and BchC extracts or alternatively a BchF extract and the purified BchC protein, with dithionite as an artificial electron donor (NAD+/NADP+ in in vivo assays), dithiothreitol, ATP, and an ATP-regenerating system (creatine phosphate and creatine phosphokinase) for the analysis of BchC (EC 1.1.1.396) substrate specificity, overview Chlorobaculum tepidum ?
-
 ?
4.2.1.165 additional information coupled enzymatic assays of BchF and BchC are initiated in an anoxic chamber, using either crude cellular BchF and BchC extracts or alternatively a BchF extract and the purified BchC protein, with dithionite as an artificial electron donor (NAD+/NADP+ in in vivo assays), dithiothreitol, ATP, and an ATP-regenerating system (creatine phosphate and creatine phosphokinase) for the analysis of BchC (EC 1.1.1.396) substrate specificity, overview Chlorobaculum tepidum ATCC 49652 / DSM 12025 / NBRC 103806 / TLS ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.165 ? x * 20008, sequence calculation, x * 25000, recombinant His6-tagged enzyme, SDS-PAGE Chlorobaculum tepidum

Synonyms

EC Number Synonyms Comment Organism
4.2.1.165 3-vinyl bacteriochlorophyllide a hydratase
-
 Chlorobaculum tepidum
4.2.1.165 bchF
-
 Chlorobaculum tepidum

General Information

EC Number General Information Comment Organism
4.2.1.165 physiological function bacteriochlorophyll a requires formation of a 3-hydroxyethyl group on pyrrole ring A that is subsequently converted into a 3-acetyl group by 3-vinyl bacteriochlorophyllide a hydratase (BchF) followed by 3-hydroxyethyl bacteriochlorophyllide a dehydrogenase (BchC) Chlorobaculum tepidum