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Literature summary extracted from

  • Zhang, Z.; Tsujimura, M.; Akutsu, J.; Sasaki, M.; Tajima, H.; Kawarabayasi, Y.
    Identification of an extremely thermostable enzyme with dual sugar-1-phosphate nucleotidylyltransferase activities from an acidothermophilic archaeon, Sulfolobus tokodaii strain 7 (2005), J. Biol. Chem., 280, 9698-9705 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.B18 expression in Escherichia coli BL21-Codon Plus (DE3)-RIL cells Sulfurisphaera tokodaii
2.7.7.B19 expression in Escherichia coli BL21-Codon Plus (DE3)-RIL cells Sulfurisphaera tokodaii
2.7.7.B20 expression in Escherichia coli BL21-Codon Plus (DE3)-RIL cells Sulfurisphaera tokodaii
2.7.7.23 expression in Escherichia coli BL21-Codon Plus (DE3)-RIL cells Sulfurisphaera tokodaii
2.7.7.24 expression in Escherichia coli BL21-Codon Plus (DE3)-RIL cells Sulfurisphaera tokodaii

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.23 additional information N-acetyl-D-glucosamine-1-phosphate uridylyltransferase activity of the mutant enzyme deletion mutant lacking the 170-residue C-terminal domain remains in the truncated enzyme after 5 min of heating at 65 °C but is completely removed by treatment over 70°C Sulfurisphaera tokodaii
2.7.7.24 additional information analysis of a deletion mutant lacking the 170-residue C-terminal domain indicated that this region has an important role in the thermostability and activity of the protein. Specific initial velocity (glucose-1-phosphate thymidylyltransferase activity) is 23 times lower than that of the native enzyme when measured at 37°C Sulfurisphaera tokodaii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.24 0.02
-
 dTTP pH 8.0, 80°C Sulfurisphaera tokodaii
2.7.7.24 0.05
-
 dTDP-alpha-D-glucose pH 8.0, 80°C Sulfurisphaera tokodaii
2.7.7.24 0.39
-
 diphosphate pH 8.0, 80°C Sulfurisphaera tokodaii
2.7.7.24 1.12
-
 alpha-D-glucose 1-phosphate pH 8.0, 80°C Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.23 Co2+ absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+ Sulfurisphaera tokodaii
2.7.7.23 Mg2+ absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+. The optimal Mg2+ concentration is 6 mM, but the enzymatic activity does not change substantially between 2 and 12 mM Sulfurisphaera tokodaii
2.7.7.23 Mn2+ absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+ Sulfurisphaera tokodaii
2.7.7.23 Zn2+ absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+ Sulfurisphaera tokodaii
2.7.7.24 Co2+ absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+ Sulfurisphaera tokodaii
2.7.7.24 Mg2+ absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+. The optimal Mg2+ concentration is 6 mM, but the enzymatic activity does not change substantially between 2 and 12 mM Sulfurisphaera tokodaii
2.7.7.24 Mn2+ absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+ Sulfurisphaera tokodaii
2.7.7.24 Zn2+ absolute requirement for a divalent cation. The order of effectiveness of metal ions on the activity of the enzyme is Co2+, Mn2+, Mg2+ and Zn2+ Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii
-
 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii DSM 16993
-
 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.7.24 dTTP + alpha-D-glucose 1-phosphate Sulfurisphaera tokodaii the enzyme is involved in biosynthesis of L-rhamnose diphosphate + dTDP-alpha-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B18 Sulfurisphaera tokodaii Q975F9
-
-
2.7.7.B18 Sulfurisphaera tokodaii DSM 16993 Q975F9
-
-
2.7.7.B19 Sulfurisphaera tokodaii Q975F9
-
-
2.7.7.B19 Sulfurisphaera tokodaii DSM 16993 Q975F9
-
-
2.7.7.B20 Sulfurisphaera tokodaii Q975F9
-
-
2.7.7.B20 Sulfurisphaera tokodaii DSM 16993 Q975F9
-
-
2.7.7.23 Sulfurisphaera tokodaii Q975F9
-
-
2.7.7.23 Sulfurisphaera tokodaii DSM 16993 Q975F9
-
-
2.7.7.24 Sulfurisphaera tokodaii Q975F9
-
-
2.7.7.24 Sulfurisphaera tokodaii DSM 16993 Q975F9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.B18
-
 Sulfurisphaera tokodaii
2.7.7.B19
-
 Sulfurisphaera tokodaii
2.7.7.B20
-
 Sulfurisphaera tokodaii
2.7.7.23
-
 Sulfurisphaera tokodaii
2.7.7.24
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.B18 dGTP + alpha-D-glucose 1-phosphate activity is 47% compared to the activity with dTTP, cf. EC 2.7.7.24 Sulfurisphaera tokodaii diphosphate + dGDP-alpha-D-glucose
-
 ?
2.7.7.B19 dGTP + alpha-D-glucose 1-phosphate activity is 47% compared to the activity with dTTP, cf. EC 2.7.7.24 Sulfurisphaera tokodaii diphosphate + dGDP-alpha-D-glucose
-
 ?
2.7.7.B20 dATP + alpha-D-glucose 1-phosphate activity is 40% compared to the activity with dTTP, cf 2.7.7.24 Sulfurisphaera tokodaii diphosphate + dADP-alpha-D-glucose
-
 ?
2.7.7.23 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 Sulfurisphaera tokodaii UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 Sulfurisphaera tokodaii DSM 16993 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 UTP + alpha-D-glucose 1-phosphate the activity with N-acetyl-D-glucosamine-1-phosphate is 3.4 times higher, respectively, than the corresponding activity with alpha-D-glucose-1-phosphate Sulfurisphaera tokodaii diphosphate + UDP-alpha-D-glucose
-
 ?
2.7.7.23 UTP + alpha-D-glucose 1-phosphate the activity with N-acetyl-D-glucosamine-1-phosphate is 3.4 times higher, respectively, than the corresponding activity with alpha-D-glucose-1-phosphate Sulfurisphaera tokodaii DSM 16993 diphosphate + UDP-alpha-D-glucose
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Sulfurisphaera tokodaii diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate the activity with N-acetyl-D-glucosamine-1-phosphate is 3.4 times higher, respectively, than the corresponding activity with alpha-D-glucose-1-phosphate Sulfurisphaera tokodaii diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 r
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 Sulfurisphaera tokodaii DSM 16993 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.7.23 UTP + N-acetyl-alpha-D-glucosamine 1-phosphate the activity with N-acetyl-D-glucosamine-1-phosphate is 3.4 times higher, respectively, than the corresponding activity with alpha-D-glucose-1-phosphate Sulfurisphaera tokodaii DSM 16993 diphosphate + UDP-N-acetyl-alpha-D-glucosamine
-
 r
2.7.7.24 diphosphate + dTDP-alpha-D-glucose
-
 Sulfurisphaera tokodaii dTTP + alpha-D-glucose 1-phosphate
-
 r
2.7.7.24 dTTP + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii diphosphate + dTDP-alpha-D-glucose
-
 r
2.7.7.24 dTTP + alpha-D-glucose 1-phosphate the enzyme is involved in biosynthesis of L-rhamnose Sulfurisphaera tokodaii diphosphate + dTDP-alpha-D-glucose
-
 ?
2.7.7.24 dTTP + alpha-D-glucose 1-phosphate
-
 Sulfurisphaera tokodaii DSM 16993 diphosphate + dTDP-alpha-D-glucose
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.B18 ST0452
-
 Sulfurisphaera tokodaii
2.7.7.B19 ST0452
-
 Sulfurisphaera tokodaii
2.7.7.B20 ST0452
-
 Sulfurisphaera tokodaii
2.7.7.23 N-acetyl-D-glucosamine-1-phosphate uridylyltransferase
-
 Sulfurisphaera tokodaii
2.7.7.23 ST0452
-
 Sulfurisphaera tokodaii
2.7.7.24 glucose-1-phosphate thymidylyltransferase
-
 Sulfurisphaera tokodaii
2.7.7.24 ST0452
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.B18 95
-
-
 Sulfurisphaera tokodaii
2.7.7.B19 95
-
-
 Sulfurisphaera tokodaii
2.7.7.B20 95
-
-
 Sulfurisphaera tokodaii
2.7.7.23 95
-
-
 Sulfurisphaera tokodaii
2.7.7.24 95
-
-
 Sulfurisphaera tokodaii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.B18 70 100 70°C: about 40% of maximal activity, 100°C: about 80% of maximal activity Sulfurisphaera tokodaii
2.7.7.B19 70 100 70°C: about 40% of maximal activity, 100°C: about 80% of maximal activity Sulfurisphaera tokodaii
2.7.7.B20 70 100 70°C: about 40% of maximal activity, 100°C: about 80% of maximal activity Sulfurisphaera tokodaii
2.7.7.23 70 100 70°C: about 40% of maximal activity, 100°C: about 80% of maximal activity Sulfurisphaera tokodaii
2.7.7.24 70 100 70°C: about 40% of maximal activity, 100°C: about 80% of maximal activity Sulfurisphaera tokodaii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.B18 80
-
 half-life: 180 min Sulfurisphaera tokodaii
2.7.7.B18 95
-
 half-life: 60 min Sulfurisphaera tokodaii
2.7.7.B19 80
-
 half-life: 180 min Sulfurisphaera tokodaii
2.7.7.B19 95
-
 half-life: 60 min Sulfurisphaera tokodaii
2.7.7.B20 80
-
 half-life: 180 min Sulfurisphaera tokodaii
2.7.7.B20 95
-
 half-life: 60 min Sulfurisphaera tokodaii
2.7.7.23 additional information
-
 N-acetyl-D-glucosamine-1-phosphate uridylyltransferase activity of the mutant enzyme deletion mutant lacking the 170-residue C-terminal domain remains in the truncated enzyme after 5 min of heating at 65 °C but is completely removed by treatment over 70°C Sulfurisphaera tokodaii
2.7.7.23 80
-
 half-life: 180 min Sulfurisphaera tokodaii
2.7.7.23 95
-
 half-life: 60 min Sulfurisphaera tokodaii
2.7.7.24 80
-
 half-life: 180 min Sulfurisphaera tokodaii
2.7.7.24 95
-
 half-life: 60 min Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.B18 8.5
-
-
 Sulfurisphaera tokodaii
2.7.7.B19 8.5
-
-
 Sulfurisphaera tokodaii
2.7.7.B20 8.5
-
-
 Sulfurisphaera tokodaii
2.7.7.23 8.5
-
-
 Sulfurisphaera tokodaii
2.7.7.24 8.5
-
-
 Sulfurisphaera tokodaii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.B18 6 10 pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity Sulfurisphaera tokodaii
2.7.7.B19 6 10 pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity Sulfurisphaera tokodaii
2.7.7.B20 6 10 pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity Sulfurisphaera tokodaii
2.7.7.23 6 10 pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity Sulfurisphaera tokodaii
2.7.7.24 6 10 pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity Sulfurisphaera tokodaii