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Literature summary extracted from

  • Hayashi, M.; Okada, A.; Yamamoto, K.; Okugochi, T.; Kusaka, C.; Kudou, D.; Nemoto, M.; Inagaki, J.; Hirose, Y.; Okajima, T.; Tamura, T.; Soda, K.; Inagaki, K.
    Gene cloning, recombinant expression, purification and characterization of L-methionine decarboxylase from Streptomyces sp. 590 (2017), J. Biochem., 161, 389-398 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.57 medicine recombinant MetDC (0.50 U/ml) severely inhibits growth of human tumour cells A431 and MDA-MB-231, but shows relatively low cytotoxicity for human normal cell NHDF-Neo Streptomyces sp. 590 KI-2014

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.57 expression in Escherichia coli Streptomyces sp. 590 KI-2014

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.57 30
-
 L-methionine pH 6.6, 55°C Streptomyces sp. 590 KI-2014
4.1.1.57 73
-
 L-norleucine pH 6.6, 55°C Streptomyces sp. 590 KI-2014

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.57 110000
-
 gel filtration Streptomyces sp. 590 KI-2014

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.57 Streptomyces sp. 590 KI-2014 A0A0G4DBU7
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.57 9
-
 pH 6.6, 55°C Streptomyces sp. 590 KI-2014

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.57 L-methionine
-
 Streptomyces sp. 590 KI-2014 3-(methylsulfanyl)propanamine + CO2
-
 ?
4.1.1.57 L-norleucine
-
 Streptomyces sp. 590 KI-2014 pentanamine + CO2
-
 ?
4.1.1.57 S-methyl-L-cysteine
-
 Streptomyces sp. 590 KI-2014 2-methylthioethanamine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.57 dimer 2 * 61000, SDS-PAGE Streptomyces sp. 590 KI-2014

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.57 45 55
-
 Streptomyces sp. 590 KI-2014

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.57 30
-
 stable below Streptomyces sp. 590 KI-2014

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.57 61
-
 L-norleucine pH 6.6, 55°C Streptomyces sp. 590 KI-2014
4.1.1.57 239
-
 L-methionine pH 6.6, 55°C Streptomyces sp. 590 KI-2014

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.57 6.6
-
-
 Streptomyces sp. 590 KI-2014

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.57 4.6 7.6
-
 Streptomyces sp. 590 KI-2014

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.57 pyridoxal 5'-phosphate
-
 Streptomyces sp. 590 KI-2014

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.57 0.83
-
 L-norleucine pH 6.6, 55°C Streptomyces sp. 590 KI-2014
4.1.1.57 8
-
 L-methionine pH 6.6, 55°C Streptomyces sp. 590 KI-2014