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Literature summary extracted from

  • Omi, R.; Mizuguchi, H.; Goto, M.; Miyahara, I.; Hayashi, H.; Kagamiyama, H.; Hirotsu, K.
    Structure of imidazole glycerol phosphate synthase from Thermus thermophilus HB8 open-closed conformational change and ammonia tunneling (2002), J. Biochem., 132, 759-765 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.10 Escherichia coli HB101 is transferred with plasmid, pUC119-hisF or pUC119-hisH. Both proteins are overexpressed in separate cells Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.2.10 crystals of subunit hisF are obtained by the hanging-drop vapor diffusion method at 20°C. The three-dimensional structure of the enzyme from Thermus thermophilus HB8 is determined at 2.3 A resolution, and compared with the structures for the yeast and Thermotoga maritima enzymes Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine Thermus thermophilus the enzyme catalyzes the step in the histidine biosynthetic pathway located at the branch point to de novo purine biosynthesis 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
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 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.10 Thermus thermophilus Q7SIC0 AND Q7SIB9 Q7SIC0: subunit HisH, Q7SIB9: subunit HisF
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Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.10
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
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 Thermus thermophilus 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?
4.3.2.10 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine the enzyme catalyzes the step in the histidine biosynthetic pathway located at the branch point to de novo purine biosynthesis Thermus thermophilus 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?

General Information

EC Number General Information Comment Organism
4.3.2.10 metabolism the enzyme catalyzes the step in the histidine biosynthetic pathway located at the branch point to de novo purine biosynthesis Thermus thermophilus