Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Watanabe, S.; Fukumori, F.; Miyazaki, M.; Tagami, S.; Watanabe, Y.
    Characterization of a novel cis-3-hydroxy-L-proline dehydratase and a trans-3-hydroxy-L-proline dehydratase from Bacteria (2017), J. Bacteriol., 199, e00255-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.77 expression in Escherichia coli BL21(DE3) Azospirillum brasilense
4.2.1.77 expression in Escherichia coli BL21(DE3) Micromonospora viridifaciens
4.2.1.171 expression in Escherichia coli BL21(DE3) Azospirillum brasilense
4.2.1.171 expression in Escherichia coli BL21(DE3) Micromonospora viridifaciens
4.2.1.171 expression in Escherichia coli BL21(DE3) Pseudomonas aeruginosa
4.2.1.171 expression in Escherichia coli BL21(DE3) Agrobacterium tumefaciens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.77 C261T the kcat/Km value is 826fold lower than that of the wild-type enzyme, and this is mainly attributed to a 162fold increase in Km Azospirillum brasilense
4.2.1.77 C261T the kcat/Km value is 826fold lower than that of the wild-type enzyme, and this is mainly attributed to a 162fold increase in Km Micromonospora viridifaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.77 0.00291
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Micromonospora viridifaciens
4.2.1.77 0.0472
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, mutant enzyme C261T Micromonospora viridifaciens
4.2.1.77 2.79
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Colwellia psychrerythraea
4.2.1.77 14.3
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Azospirillum brasilense
4.2.1.171 0.21
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 0.387
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 0.387
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Pseudomonas aeruginosa
4.2.1.171 0.531
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Azospirillum brasilense
4.2.1.171 0.531
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 0.634
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 0.634
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.77 trans-3-hydroxy-L-proline Azospirillum brasilense
-
 1-pyrroline 2-carboxylate + H2O
-
 ?
4.2.1.77 trans-3-hydroxy-L-proline Colwellia psychrerythraea
-
 1-pyrroline 2-carboxylate + H2O
-
 ?
4.2.1.77 trans-3-hydroxy-L-proline Micromonospora viridifaciens
-
 1-pyrroline 2-carboxylate + H2O
-
 ?
4.2.1.77 trans-3-hydroxy-L-proline Azospirillum brasilense NBRC 102289
-
 1-pyrroline 2-carboxylate + H2O
-
 ?
4.2.1.171 cis-3-hydroxy-L-proline Azospirillum brasilense
-
 1-pyrroline-2-carboxylate + H2O
-
 ?
4.2.1.171 cis-3-hydroxy-L-proline Azospirillum brasilense NBRC 102289
-
 1-pyrroline-2-carboxylate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.77 Azospirillum brasilense
-
-
-
4.2.1.77 Azospirillum brasilense NBRC 102289
-
-
-
4.2.1.77 Colwellia psychrerythraea Q485S0
-
-
4.2.1.77 Micromonospora viridifaciens A0A1C4WR37 cf. EC 5.1.1.4
-
4.2.1.171 Agrobacterium tumefaciens A9CH00
-
-
4.2.1.171 Agrobacterium tumefaciens C58 A9CH00
-
-
4.2.1.171 Azospirillum brasilense A0A1S7J048 AND A0A1S7J047 A0A1S7J048: cis-3-hydroxy-L-proline dehydratase small subunit, A0A1S7J047: cis-3-hydroxy-L-proline dehydratase large subunit
-
4.2.1.171 Azospirillum brasilense NBRC 102289 A0A1S7J048 AND A0A1S7J047 A0A1S7J048: cis-3-hydroxy-L-proline dehydratase small subunit, A0A1S7J047: cis-3-hydroxy-L-proline dehydratase large subunit
-
4.2.1.171 Micromonospora viridifaciens A0A1C4WQP5 AND A0A1C4WQE0 A0A1C4WQP5: cis-3-hydroxy-L-proline dehydratase small subunit, A0A1C4WQE0: cis-3-hydroxy-L-proline dehydratase large subunit
-
4.2.1.171 Pseudomonas aeruginosa Q9I485
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.77
-
 Azospirillum brasilense
4.2.1.77
-
 Micromonospora viridifaciens
4.2.1.171
-
 Azospirillum brasilense
4.2.1.171
-
 Micromonospora viridifaciens
4.2.1.171
-
 Pseudomonas aeruginosa
4.2.1.171
-
 Agrobacterium tumefaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.77 0.877
-
 substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, mutant enzyme C261T Micromonospora viridifaciens
4.2.1.77 17.6
-
 substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, wild-type enzyme Micromonospora viridifaciens
4.2.1.77 25.4
-
 substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, wild-type enzyme Colwellia psychrerythraea
4.2.1.77 26.1
-
 substrate: trans-3-hydroxy-L-proline, pH 8.0, 30°C, wild-type enzyme Azospirillum brasilense
4.2.1.171 2.29
-
 substrate: cis-3-hydroxy-L-proline, pH 8.0, 30°C Azospirillum brasilense
4.2.1.171 2.29
-
 substrate: cis-3-hydroxy-L-proline, pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 2.34
-
 substrate: cis-3-hydroxy-L-proline, pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 18.2
-
 substrate: cis-3-hydroxy-L-proline, pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 18.2
-
 substrate: cis-3-hydroxy-L-proline, pH 8.0, 30°C Agrobacterium tumefaciens
4.2.1.171 41.3
-
 substrate: cis-3-hydroxy-L-proline, pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 41.3
-
 substrate: cis-3-hydroxy-L-proline, pH 8.0, 30°C Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.77 trans-3-hydroxy-L-proline
-
 Azospirillum brasilense 1-pyrroline 2-carboxylate + H2O
-
 ?
4.2.1.77 trans-3-hydroxy-L-proline
-
 Colwellia psychrerythraea 1-pyrroline 2-carboxylate + H2O
-
 ?
4.2.1.77 trans-3-hydroxy-L-proline
-
 Micromonospora viridifaciens 1-pyrroline 2-carboxylate + H2O
-
 ?
4.2.1.77 trans-3-hydroxy-L-proline
-
 Azospirillum brasilense NBRC 102289 1-pyrroline 2-carboxylate + H2O
-
 ?
4.2.1.171 cis-3-hydroxy-L-proline
-
 Azospirillum brasilense 1-pyrroline-2-carboxylate + H2O
-
 ?
4.2.1.171 cis-3-hydroxy-L-proline
-
 Micromonospora viridifaciens 1-pyrroline-2-carboxylate + H2O
-
 ?
4.2.1.171 cis-3-hydroxy-L-proline
-
 Pseudomonas aeruginosa 1-pyrroline-2-carboxylate + H2O
-
 ?
4.2.1.171 cis-3-hydroxy-L-proline
-
 Agrobacterium tumefaciens 1-pyrroline-2-carboxylate + H2O
-
 ?
4.2.1.171 cis-3-hydroxy-L-proline
-
 Azospirillum brasilense NBRC 102289 1-pyrroline-2-carboxylate + H2O
-
 ?
4.2.1.171 cis-3-hydroxy-L-proline
-
 Agrobacterium tumefaciens C58 1-pyrroline-2-carboxylate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.171 heterodimer
-
 Azospirillum brasilense
4.2.1.171 heterodimer
-
 Micromonospora viridifaciens

Synonyms

EC Number Synonyms Comment Organism
4.2.1.77 AbHypG
-
 Azospirillum brasilense
4.2.1.77 bifunctional T3LHyp dehydratase/2-epimerase
-
 Micromonospora viridifaciens
4.2.1.77 CpHypG
-
 Colwellia psychrerythraea
4.2.1.77 MvHypA2
-
 Micromonospora viridifaciens
4.2.1.171 AcnX
-
 Micromonospora viridifaciens
4.2.1.171 AcnX
-
 Pseudomonas aeruginosa
4.2.1.171 AcnX
-
 Agrobacterium tumefaciens
4.2.1.171 AcnX type IIa
-
 Azospirillum brasilense
4.2.1.171 C3LHyp dehydratase
-
 Azospirillum brasilense
4.2.1.171 C3LHyp dehydratase
-
 Micromonospora viridifaciens
4.2.1.171 C3LHyp dehydratase
-
 Pseudomonas aeruginosa
4.2.1.171 C3LHyp dehydratase
-
 Agrobacterium tumefaciens
4.2.1.171 cis-3-hydroxy-L-proline dehydratase
-
 Azospirillum brasilense
4.2.1.171 cis-3-hydroxy-L-proline dehydratase
-
 Micromonospora viridifaciens
4.2.1.171 cis-3-hydroxy-L-proline dehydratase
-
 Pseudomonas aeruginosa
4.2.1.171 cis-3-hydroxy-L-proline dehydratase
-
 Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.77 2.43
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, mutant enzyme C261T Micromonospora viridifaciens
4.2.1.77 3.4
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Micromonospora viridifaciens
4.2.1.77 22.17
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Colwellia psychrerythraea
4.2.1.77 37.33
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Azospirillum brasilense
4.2.1.171 2.08
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 2.4
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Azospirillum brasilense
4.2.1.171 2.4
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 20
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 20
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Agrobacterium tumefaciens
4.2.1.171 47
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 47
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
4.2.1.171 metabolism a strain disrupted in the hypIS gene does not grow on C3LHyp, suggesting its involvement in C3LHyp metabolism Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.77 0.052
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, mutant enzyme C261T Micromonospora viridifaciens
4.2.1.77 2.62
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Azospirillum brasilense
4.2.1.77 7.95
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Colwellia psychrerythraea
4.2.1.77 42.7
-
 trans-3-hydroxy-L-proline pH 8.0, 30°C, wild-type enzyme Micromonospora viridifaciens
4.2.1.171 4.5
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Azospirillum brasilense
4.2.1.171 4.5
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 9.93
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 31.7
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 31.7
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Agrobacterium tumefaciens
4.2.1.171 122.17
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Micromonospora viridifaciens
4.2.1.171 122.17
-
 cis-3-hydroxy-L-proline pH 8.0, 30°C Pseudomonas aeruginosa