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Literature summary extracted from

  • Cech, D.; Markin, K.; Woodard, R.
    Identification of a D-arabinose-5-phosphate isomerase in the Gram-positive Clostridium tetani (2017), J. Bacteriol., 199, e00246 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.13 expression in Escherichia coli Clostridium tetani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.13 1.89
-
 D-arabinose 5-phosphate pH 6.5, 37°C Clostridium tetani
5.3.1.13 6.65
-
 D-ribulose 5-phosphate pH 6.5, 37°C Clostridium tetani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.13 109200
-
 gel filtration Clostridium tetani

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.13 Clostridium tetani A0A4Q0VE60
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.13 D-Arabinose 5-phosphate
-
 Clostridium tetani D-Ribulose 5-phosphate
-
 ?
5.3.1.13 D-ribulose 5-phosphate
-
 Clostridium tetani D-arabinose 5-phosphate
-
 ?
5.3.1.13 additional information no substrates: D-arabinose, D-arabinose-5-phosphate, D-glucose-6-phosphate, D-mannose-6-phosphate, D-glucosamine-6-phosphate, D-glucose-1-phosphate, and D-ribose-5-phosphate Clostridium tetani ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.13 tetramer 4 * 26000, SDS-PAGE, His-tagged recombinant protein, x * 25740, calculated from sequence Clostridium tetani

Synonyms

EC Number Synonyms Comment Organism
5.3.1.13 API
-
 Clostridium tetani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.13 86.7
-
 D-ribulose 5-phosphate pH 6.5, 37°C Clostridium tetani
5.3.1.13 102
-
 D-arabinose 5-phosphate pH 6.5, 37°C Clostridium tetani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.13 6 8 broad Clostridium tetani

General Information

EC Number General Information Comment Organism
5.3.1.13 physiological function expression complements the API deficiency of an Escherichia coli API knockout strain Clostridium tetani

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.13 13
-
 D-ribulose 5-phosphate pH 6.5, 37°C Clostridium tetani
5.3.1.13 54
-
 D-arabinose 5-phosphate pH 6.5, 37°C Clostridium tetani