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Literature summary extracted from

  • Ruscitto, A.; Hottmann, I.; Stafford, G.P.; Schaeffer, C.; Mayer, C.; Sharma, A.
    Identification of a novel N-acetylmuramic acid transporter in Tannerella forsythia (2016), J. Bacteriol., 198, 3119-3125 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.126 gene murQ, DNA and amino acid sequence determination and analysis, genetic organization of the MurNAc utilization locus, the three genes, Tanf_08375, Tanf_08380, and Tanf_08385 (murQ), encoding a MurNAc transporter, a putative sugar kinase, and a MurQ etherase, respectively, are transcriptionally linked. Complementation with the Tanf_08375 and Tanf_08380 genes together in trans, but not individually, rescues the inability of an Escherichia coli mutant deficient in the phosphotransferase (PTS) system-dependent MurNAc transporter MurP as well as that of a double mutant deficient in MurP and components of the PTS system to grow on MurNAc. In addition, complementation with this two-gene construct in Escherichia coli causes depletion of MurNAc in the medium. Recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Tannerella forsythia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.126 (R)-lactate + N-acetyl-D-glucosamine 6-phosphate Tannerella forsythia
-
 N-acetylmuramate 6-phosphate + H2O
-
 r
4.2.1.126 (R)-lactate + N-acetyl-D-glucosamine 6-phosphate Tannerella forsythia ATCC 43037 / JCM 10827 / FDC 338
-
 N-acetylmuramate 6-phosphate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.126 Tannerella forsythia G8UQI0 i.e. Bacteroides forsythus
-
4.2.1.126 Tannerella forsythia ATCC 43037 / JCM 10827 / FDC 338 G8UQI0 i.e. Bacteroides forsythus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.126 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Tannerella forsythia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.126 (R)-lactate + N-acetyl-D-glucosamine 6-phosphate
-
 Tannerella forsythia N-acetylmuramate 6-phosphate + H2O
-
 r
4.2.1.126 (R)-lactate + N-acetyl-D-glucosamine 6-phosphate
-
 Tannerella forsythia ATCC 43037 / JCM 10827 / FDC 338 N-acetylmuramate 6-phosphate + H2O
-
 r
4.2.1.126 additional information the etherase activity is assessed by utilizing N-acetylmuramate 6-phosphate as the substrate in a Morgan Elson reaction. This etherase-catalyzed reaction generates a chromogenic intermediate that can be detected by reacting with Ehrlich's reagent dimethylaminobenzaldehyde to yield a purple product. Also, radioactive assay with 32P-radiolabeled substrates N-acetyl-D-glucosamine 6-phosphate and N-acetylmuramate 6-phosphate Tannerella forsythia ?
-
 ?
4.2.1.126 additional information the etherase activity is assessed by utilizing N-acetylmuramate 6-phosphate as the substrate in a Morgan Elson reaction. This etherase-catalyzed reaction generates a chromogenic intermediate that can be detected by reacting with Ehrlich's reagent dimethylaminobenzaldehyde to yield a purple product. Also, radioactive assay with 32P-radiolabeled substrates N-acetyl-D-glucosamine 6-phosphate and N-acetylmuramate 6-phosphate Tannerella forsythia ATCC 43037 / JCM 10827 / FDC 338 ?
-
 ?
4.2.1.126 N-acetylmuramate 6-phosphate + H2O
-
 Tannerella forsythia (R)-lactate + N-acetyl-D-glucosamine 6-phosphate
-
 r
4.2.1.126 N-acetylmuramate 6-phosphate + H2O
-
 Tannerella forsythia ATCC 43037 / JCM 10827 / FDC 338 (R)-lactate + N-acetyl-D-glucosamine 6-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.126 MurQ
-
 Tannerella forsythia
4.2.1.126 MurQ etherase
-
 Tannerella forsythia
4.2.1.126 Tanf_08385
-
 Tannerella forsythia
4.2.1.126 TfMurQ
-
 Tannerella forsythia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.126 37 45 assay at Tannerella forsythia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.126 7.2 7.6 assay at Tannerella forsythia

General Information

EC Number General Information Comment Organism
4.2.1.126 metabolism MurNAc utilization locus and MurNAc utilization pathway in Tannerella forsythia, overview. The three genes, Tanf_08375, Tanf_08380, and Tanf_08385 (murQ), encoding a MurNAc transporter, a putative sugar kinase, and a MurQ etherase, respectively, are transcriptionally linked. Enzymes MurT-MurK function as a PTS-independent MurNAc transporter Tannerella forsythia
4.2.1.126 physiological function the enzyme is involved in the metabolic conversion of MurNAc-6-phosphate to GlcNAc-6-phosphate Tannerella forsythia