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Literature summary extracted from

  • Braune, A.; Engst, W.; Elsinghorst, P.W.; Furtmann, N.; Bajorath, J.; Guetschow, M.; Blaut, M.
    Chalcone isomerase from Eubacterium ramulus catalyzes the ring contraction of flavanonols (2016), J. Bacteriol., 198, 2965-2974 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.6 expressed in Escherichia coli JM109 cells Eubacterium ramulus

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.6 H33A inactive Eubacterium ramulus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.6 0.0041
-
 (+)-taxifolin at pH 6.8 and 23°C Eubacterium ramulus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.5.1.6 cytosol
-
 Eubacterium ramulus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.6 270000
-
 gel filtration Eubacterium ramulus

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.6 Eubacterium ramulus V9P0A9
-
-
5.5.1.6 Eubacterium ramulus DSM 16296 V9P0A9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.6 ammonium sulfate precipitation, DEAE-Sephacel column chromatography, phenyl Sepharose column chromatography, Mono Q column chromatography, Superdex 200 gel filtration, and phenyl Superose gel filtration Eubacterium ramulus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.6 (+)-dihydrokaempferol 60% activity compared to (+)-taxifolin Eubacterium ramulus maesopsin
-
 ?
5.5.1.6 (+)-dihydrokaempferol 60% activity compared to (+)-taxifolin Eubacterium ramulus DSM 16296 maesopsin
-
 ?
5.5.1.6 (+)-taxifolin
-
 Eubacterium ramulus alphitonin
-
 ?
5.5.1.6 (+)-taxifolin
-
 Eubacterium ramulus DSM 16296 alphitonin
-
 ?
5.5.1.6 butein less than 1% activity compared to (+)-taxifolin Eubacterium ramulus (S)-eriodictyol
-
 ?
5.5.1.6 butein less than 1% activity compared to (+)-taxifolin Eubacterium ramulus DSM 16296 (S)-eriodictyol
-
 ?
5.5.1.6 additional information no activity with (-)-taxifolin, (-)-dihydrokaempferol, naringenin, or eriodictyol Eubacterium ramulus ?
-
 ?
5.5.1.6 additional information no activity with (-)-taxifolin, (-)-dihydrokaempferol, naringenin, or eriodictyol Eubacterium ramulus DSM 16296 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.6 ? x * 31000, SDS-PAGE Eubacterium ramulus
5.5.1.6 ? x * 32448, calculated from amino acid sequence Eubacterium ramulus

Synonyms

EC Number Synonyms Comment Organism
5.5.1.6 CHI
-
 Eubacterium ramulus
5.5.1.6 taxifolin-isomerizing enzyme
-
 Eubacterium ramulus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.5.1.6 41
-
-
 Eubacterium ramulus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.5.1.6 5.2 8
-
 Eubacterium ramulus