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Literature summary extracted from

  • Palmer, L.D.; Paxhia, M.D.; Downs, D.M.
    Induction of the sugar-phosphate stress response allows Saccharomyces cerevisiae 2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate synthase to function in Salmonella enterica (2015), J. Bacteriol., 197, 3554-3562 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.99.17 additional information one of the mechanisms of Thi5p activation requires decreased PtsG function and an undefined role of SgrR Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.17 gene YFL058w, recombinant expression of GST-tagged enzyme mutant in Salmonella enterica strain LT2, one mechanism to allow Thi5p function in Salmonella enterica is by remodeling the metabolic network associated with the sugar phosphate stress response regulator, transcription factor SgrR (formerly YabN), integration between the sugar-phosphate stress response regulator and Thi5p activity in Salmonella enterica. SgrR belongs to a distinct class of transcription regulators (COG4533) and has a predicted N-terminal DNA-binding domain and C-terminal solute-binding domain, expression from the sgrS promoter (sgrSp) is used as a reporter of SgrR activity. Thi5p must be activated in order to function in Salmonella enterica, possibly by phosphorylation or another posttranslational modification Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.17 M37I/A138V/G152D site-directed mutagenesis Saccharomyces cerevisiae
4.1.99.17 additional information disruption of glycolysis allows Thi5p-dependent growth on glucose. Generation of an insertion mutation in ptsG allowing Thi5p-dependent thiamine synthesis in a DELTAthiC strain, the effect of the ptsG mutation is independent of sgrS, consistent with a role for sgrS in decreasing PtsG activity. Induction of the sugar-phosphate stress response is required to decrease PtsG activity. Disrupting ptsG independently restores thiamine synthesis revealing one mechanism connecting Thi5p function and induction of the sugar-phosphate stress response Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine Saccharomyces cerevisiae
-
 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine Saccharomyces cerevisiae ATCC 204508 / S288c
-
 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.17 Saccharomyces cerevisiae P43534
-
-
4.1.99.17 Saccharomyces cerevisiae ATCC 204508 / S288c P43534
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine
-
 Saccharomyces cerevisiae 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?
4.1.99.17 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine
-
 Saccharomyces cerevisiae ATCC 204508 / S288c 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.99.17 2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate synthase
-
 Saccharomyces cerevisiae
4.1.99.17 HMP-P synthase
-
 Saccharomyces cerevisiae
4.1.99.17 Thi5p
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.17 S-adenosyl-L-methionine
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
4.1.99.17 malfunction one mechanism to allow Thi5p function in Salmonella enterica is by remodeling the metabolic network associated with the sugar phosphate stress response regulator, transcription factor SgrR (formerly YabN), integration between the sugar-phosphate stress response regulator and Thi5p activity in Salmonella enterica. SgrR belongs to a distinct class of transcription regulators (COG4533) and has a predicted N-terminal DNA-binding domain and C-terminal solute-binding domain, expression from the sgrS promoter (sgrSp) is used as a reporter of SgrR activity Saccharomyces cerevisiae
4.1.99.17 metabolism one of the mechanisms of Thi5p activation requires decreased PtsG function and an undefined role of SgrR Saccharomyces cerevisiae