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Literature summary extracted from

  • Oh, S.Y.; Richter, S.G.; Missiakas, D.M.; Schneewind, O.
    Glutamate racemase mutants of Bacillus anthracis (2015), J. Bacteriol., 197, 1854-1861 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.3 identification of two insertions in gene racE1, suggesting that its glutamate racemase product is not essential for Bacillus anthracis growth Bacillus anthracis

Protein Variants

EC Number Protein Variants Comment Organism
5.1.1.3 additional information using allelic replacement, chromosomal racE2 is replaced with the spectinomycin resistance gene (aad9) and transduced via bacteriophage CP-51 into Bacillus anthracis strain Sterne, generating strain SYO2 (DELTAracE2::aad9) Bacillus anthracis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.3 L-glutamate Bacillus anthracis
-
 D-glutamate
-
 r
5.1.1.3 L-glutamate Bacillus anthracis Sterne 34F2
-
 D-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.3 Bacillus anthracis Q81LA8
-
-
5.1.1.3 Bacillus anthracis Q81UL8
-
-
5.1.1.3 Bacillus anthracis Sterne 34F2 Q81LA8
-
-
5.1.1.3 Bacillus anthracis Sterne 34F2 Q81UL8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.3 L-glutamate
-
 Bacillus anthracis D-glutamate
-
 r
5.1.1.3 L-glutamate
-
 Bacillus anthracis Sterne 34F2 D-glutamate
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.1.3 BAS0806
-
 Bacillus anthracis
5.1.1.3 BAS4379
-
 Bacillus anthracis
5.1.1.3 GBAA_0847
-
 Bacillus anthracis
5.1.1.3 GBAA_4717
-
 Bacillus anthracis
5.1.1.3 MurI
-
 Bacillus anthracis
5.1.1.3 RacE1
-
 Bacillus anthracis
5.1.1.3 RacE2
-
 Bacillus anthracis

General Information

EC Number General Information Comment Organism
5.1.1.3 malfunction the racE1 deletion does not affect growth rates, whereas racE2 deletion delays the vegetative growth of Bacillus anthracis following spore germination, producing aberrant cell shapes that can only partially be suppressed with exogenous D-glutamate. Deletion of racE1 or racE2 does not affect the synthesis or stereochemical composition of Bacillus anthracis poly-gamma-D-glutamic acid (PDGA) capsule, and mutants with a deletion of both genes, racE1 and racE2, are not viable Bacillus anthracis
5.1.1.3 physiological function genes racE1 and racE2, encoding two glutamate racemases, are not essential for growth of Bacillus anthracis noncapsulating vaccine strain Sterne and fully virulent, capsulating strain Ames, even in the absence of exogenous D-glutamate. Modeling of Bacillus anthracis, similar to Bacillus subtilis, utilizing two functionally redundant racemase enzymes to synthesize D-glutamic acid for peptidoglycan synthesis Bacillus anthracis
5.1.1.3 physiological function genes racE1 and racE2, encoding two glutamate racemases,are not essential for growth of Bacillus anthracis noncapsulating vaccine strain Sterne and fully virulent, capsulating strain Ames, even in the absence of exogenous D-glutamate. Modeling of Bacillus anthracis, similar to Bacillus subtilis, utilizing two functionally redundant racemase enzymes to synthesize D-glutamic acid for peptidoglycan synthesis Bacillus anthracis