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Literature summary extracted from

  • Yang, Y.; Yatsunami, R.; Ando, A.; Miyoko, N.; Fukui, T.; Takaichi, S.; Nakamura, S.
    Complete biosynthetic pathway of the C50 carotenoid bacterioruberin from lycopene in the extremely halophilic archaeon Haloarcula japonica (2015), J. Bacteriol., 197, 1614-1623 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.161 gene cruF or c0505, functional complementation of enzyme-deficient mutant DELTAc0505 by expression of the intact gene c0505 Haloarcula japonica

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.161 additional information construction of an enzyme deletion DELTAc0505 mutant strain, the mutant is constructed by homologous recombination. The transcriptions of genes c0507 and c0506 in the DELTAc0505 strain are confirmed by RT-PCR, and the disruption of c0505 does not affect the expression of the upstream genes in the same cluster. In vivo complementation using intact c0505 gene introduced into transformant DELTAc0505(pJc0505), the carotenoid composition of the DELTAc0505(pJc0505) transformant is restored and similar to that of the wild-type Haloarcula japonica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.150 dimethylallyl diphosphate + all-trans-lycopene + H2O Haloarcula japonica the enzyme is involved in the biosynthetic pathway of the C50 carotenoid bacterioruberin. The enzyme is bifunctional. It catalyses the elongation of the C40 carotenoid all-trans-lycopene by attaching an isoprene unit at C-2 as well as the hydroxylation of the previous end of the molecule. The enzyme acts at both ends of the substrate dihydroisopentenyldehydrorhodopin + diphosphate
-
 ?
2.5.1.150 dimethylallyl diphosphate + isopentenyldehydrorhodopin + H2O Haloarcula japonica the enzyme is involved in the biosynthetic pathway of the C50 carotenoid bacterioruberin. The enzyme is bifunctional. It catalyses the elongation of the C40 carotenoid all-trans-lycopene by attaching an isoprene unit at C-2 as well as the hydroxylation of the previous end of the molecule. The enzyme acts at both ends of the substrate dihydrobisanhydrobacterioruberin + diphosphate
-
 ?
4.2.1.161 bacterioruberin Haloarcula japonica overall reaction bisanhydrobacterioruberin + 2 H2O
-
 r
4.2.1.161 bacterioruberin Haloarcula japonica
-
 monoanhydrobacterioruberin + H2O
-
 r
4.2.1.161 bacterioruberin Haloarcula japonica JCM 7785 overall reaction bisanhydrobacterioruberin + 2 H2O
-
 r
4.2.1.161 bacterioruberin Haloarcula japonica JCM 7785
-
 monoanhydrobacterioruberin + H2O
-
 r
4.2.1.161 monoanhydrobacterioruberin Haloarcula japonica
-
 bisanhydrobacterioruberin + H2O
-
 r
4.2.1.161 monoanhydrobacterioruberin Haloarcula japonica JCM 7785
-
 bisanhydrobacterioruberin + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.150 Haloarcula japonica A0A0A1GNW8
-
-
4.2.1.161 Haloarcula japonica A0A0A1GNF2
-
-
4.2.1.161 Haloarcula japonica JCM 7785 A0A0A1GNF2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.150 dimethylallyl diphosphate + all-trans-lycopene + H2O the enzyme is bifunctional. It catalyses the elongation of the C40 carotenoid all-trans-lycopene by attaching an isoprene unit at C-2 as well as the hydroxylation of the previous end of the molecule. The enzyme acts at both ends of the substrate Haloarcula japonica dihydroisopentenyldehydrorhodopin + diphosphate
-
 ?
2.5.1.150 dimethylallyl diphosphate + all-trans-lycopene + H2O the enzyme is involved in the biosynthetic pathway of the C50 carotenoid bacterioruberin. The enzyme is bifunctional. It catalyses the elongation of the C40 carotenoid all-trans-lycopene by attaching an isoprene unit at C-2 as well as the hydroxylation of the previous end of the molecule. The enzyme acts at both ends of the substrate Haloarcula japonica dihydroisopentenyldehydrorhodopin + diphosphate
-
 ?
2.5.1.150 dimethylallyl diphosphate + isopentenyldehydrorhodopin + H2O the enzyme is bifunctional. It catalyses the elongation of the C40 carotenoid all-trans-lycopene by attaching an isoprene unit at C-2 as well as the hydroxylation of the previous end of the molecule. The enzyme acts at both ends of the substrate Haloarcula japonica dihydrobisanhydrobacterioruberin + diphosphate
-
 ?
2.5.1.150 dimethylallyl diphosphate + isopentenyldehydrorhodopin + H2O the enzyme is involved in the biosynthetic pathway of the C50 carotenoid bacterioruberin. The enzyme is bifunctional. It catalyses the elongation of the C40 carotenoid all-trans-lycopene by attaching an isoprene unit at C-2 as well as the hydroxylation of the previous end of the molecule. The enzyme acts at both ends of the substrate Haloarcula japonica dihydrobisanhydrobacterioruberin + diphosphate
-
 ?
4.2.1.161 bacterioruberin overall reaction Haloarcula japonica bisanhydrobacterioruberin + 2 H2O
-
 r
4.2.1.161 bacterioruberin overall reaction Haloarcula japonica JCM 7785 bisanhydrobacterioruberin + 2 H2O
-
 r
4.2.1.161 bacterioruberin
-
 Haloarcula japonica monoanhydrobacterioruberin + H2O
-
 r
4.2.1.161 bacterioruberin
-
 Haloarcula japonica JCM 7785 monoanhydrobacterioruberin + H2O
-
 r
4.2.1.161 monoanhydrobacterioruberin
-
 Haloarcula japonica bisanhydrobacterioruberin + H2O
-
 r
4.2.1.161 monoanhydrobacterioruberin
-
 Haloarcula japonica JCM 7785 bisanhydrobacterioruberin + H2O
-
 r
4.2.1.161 additional information enzyme C0505 catalyzes the reaction that introduces hydroxyl groups to C3'' and C3''' Haloarcula japonica ?
-
 ?
4.2.1.161 additional information enzyme C0505 catalyzes the reaction that introduces hydroxyl groups to C3'' and C3''' Haloarcula japonica JCM 7785 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.150 c0506
-
 Haloarcula japonica
2.5.1.150 lycopene elongase/1,2-hydratase bifunctional enzyme Haloarcula japonica
2.5.1.150 lyeJ
-
 Haloarcula japonica
4.2.1.161 c0505
-
 Haloarcula japonica
4.2.1.161 C50 carotenoid 2'',3''-hydratase
-
 Haloarcula japonica
4.2.1.161 CruF
-
 Haloarcula japonica

General Information

EC Number General Information Comment Organism
2.5.1.150 metabolism the enzyme is involved in the biosynthetic pathway of the C50 carotenoid bacterioruberin Haloarcula japonica
4.2.1.161 evolution C0505, the C50 carotenoid 2'',3''-hydratase from Haloarcula japonica, is a homologue of the CruF-type carotenoid 1,2-hydratase Haloarcula japonica
4.2.1.161 metabolism the gene cluster, including genes c0507, c0506, and c0505, is involved in the synthesis of bacterioruberin. Genes c0507, c0506, and c0505 encode a carotenoid 3,4-desaturase (CrtD), a bifunctional lycopene elongase and 1,2-hydratase (LyeJ), and a C50 carotenoid 2'',3''-hydratase (CruF), respectively. The three carotenoid biosynthetic enzymes catalyze the reactions that convert lycopene to bacterioruberin in Haloarcula japonica. Biosynthetic pathway of C50 carotenoids, overview Haloarcula japonica