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Literature summary extracted from

  • Glenn, K.; Smith, K.S.
    Allosteric regulation of Lactobacillus plantarum xylulose 5-phosphate/fructose 6-phosphate phosphoketolase (Xfp) (2015), J. Bacteriol., 197, 1157-1163 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.22 expressed in Escherichia coli BL21(DE3) cells Lactiplantibacillus plantarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.22 glyoxylate 7.2% residual activity at 8 mM Lactiplantibacillus plantarum
4.1.2.22 additional information the enzyme is unaffected by the presence of ATP, AMP, pyruvate, and acetate Lactiplantibacillus plantarum
4.1.2.22 oxaloacetic acid about 30% residual activity at 15 mM Lactiplantibacillus plantarum
4.1.2.22 phosphoenolpyruvate less than 20% residual activity at 15 mM Lactiplantibacillus plantarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.22 10.8
-
 D-fructose 6-phosphate at pH 6.0 and 37°C Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.22 D-Fructose 6-phosphate + phosphate Lactiplantibacillus plantarum
-
 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
4.1.2.22 D-Xylulose 5-phosphate + phosphate Lactiplantibacillus plantarum
-
 Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.22 Lactiplantibacillus plantarum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.22 His-Trap column chromatography Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.22 D-Fructose 6-phosphate + phosphate
-
 Lactiplantibacillus plantarum Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
4.1.2.22 D-Xylulose 5-phosphate + phosphate
-
 Lactiplantibacillus plantarum Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.22 Xfp bifunctional enzyme with xylulose 5-phosphate and fructose 6-phosphate phosphoketolase activities Lactiplantibacillus plantarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.22 1.05
-
 D-fructose 6-phosphate at pH 6.0 and 37°C Lactiplantibacillus plantarum
4.1.2.22 1.12
-
 phosphate at pH 6.0 and 37°C Lactiplantibacillus plantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.22 5.5
-
-
 Lactiplantibacillus plantarum

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.22 thiamine diphosphate
-
 Lactiplantibacillus plantarum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.2.22 1.93
-
 at pH 6.0 and 37°C Lactiplantibacillus plantarum glyoxylate
4.1.2.22 6.7
-
 at pH 6.0 and 37°C Lactiplantibacillus plantarum phosphoenolpyruvate
4.1.2.22 10.5
-
 at pH 6.0 and 37°C Lactiplantibacillus plantarum oxaloacetic acid

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.22 0.1
-
 D-fructose 6-phosphate at pH 6.0 and 37°C Lactiplantibacillus plantarum
4.1.2.22 1.11
-
 phosphate at pH 6.0 and 37°C Lactiplantibacillus plantarum