Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, Y.; Xu, H.; Grochowski, L.L.; White, R.H.
    Biochemical characterization of a dihydroneopterin aldolase used for methanopterin biosynthesis in methanogens (2014), J. Bacteriol., 196, 3191-3198 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.25 E25Q the mutation causes a more than 25fold increase in Km value Methanocaldococcus jannaschii
4.1.2.25 H35N the mutant shows an about 20fold decreases in kcat value Methanocaldococcus jannaschii
4.1.2.25 Q61A the mutant shows an about 20fold decreases in kcat value Methanocaldococcus jannaschii
4.1.2.25 Y111F the mutation causes a 40% decrease in kcat value Methanocaldococcus jannaschii
4.1.2.25 Y78F the mutant shows an about 20fold decreases in kcat value Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.25 additional information not inhibited by EDTA Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.25 0.0084
-
7,8-dihydroneopterin mutant enzyme Q61A, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 0.0099
-
7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 0.011
-
7,8-dihydroneopterin mutant enzyme Y111F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 0.021
-
7,8-dihydroneopterin mutant enzyme H35N, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 0.03
-
7,8-dihydroneopterin mutant enzyme Y78F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 0.0645
-
7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 25°C Methanocaldococcus jannaschii
4.1.2.25 0.25
-
7,8-dihydroneopterin Km above 0.25 mM, mutant enzyme E25Q, at pH 7.6 and 70°C Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.25 additional information the metal-independent enzyme is not influenced by Fe2+, Mn2+, Cu2+, Co2+, Ni2+, Mg2+, or Zn2+ Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.25 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde Methanocaldococcus jannaschii
-
7,8-dihydroneopterin + 7,8-dihydromonapterin
-
r
4.1.2.25 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde Methanocaldococcus jannaschii MJ0408
-
7,8-dihydroneopterin + 7,8-dihydromonapterin
-
r
4.1.2.25 7,8-dihydroneopterin Methanocaldococcus jannaschii
-
6-(hydroxymethyl)-7,8-dihydropterin + glycolaldehyde
-
r
4.1.2.25 7,8-dihydroneopterin Methanocaldococcus jannaschii MJ0408
-
6-(hydroxymethyl)-7,8-dihydropterin + glycolaldehyde
-
r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.25 Methanocaldococcus jannaschii
-
-
-
4.1.2.25 Methanocaldococcus jannaschii MJ0408
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.25 MonoQ anion exchange column chromatography and gel filtration Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.25 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
Methanocaldococcus jannaschii 7,8-dihydroneopterin + 7,8-dihydromonapterin
-
r
4.1.2.25 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
-
Methanocaldococcus jannaschii MJ0408 7,8-dihydroneopterin + 7,8-dihydromonapterin
-
r
4.1.2.25 7,8-dihydroneopterin
-
Methanocaldococcus jannaschii 6-(hydroxymethyl)-7,8-dihydropterin + glycolaldehyde
-
r
4.1.2.25 7,8-dihydroneopterin
-
Methanocaldococcus jannaschii MJ0408 6-(hydroxymethyl)-7,8-dihydropterin + glycolaldehyde
-
r

Subunits

EC Number Subunits Comment Organism
4.1.2.25 ? x * 14000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
4.1.2.25 DHNA
-
Methanocaldococcus jannaschii
4.1.2.25 MptD
-
Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.25 80
-
the enzyme retains its activity following heating at 80°C for 20 min Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.25 0.048
-
7,8-dihydroneopterin mutant enzyme Q61A, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 0.054
-
7,8-dihydroneopterin mutant enzyme Y78F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 0.055
-
7,8-dihydroneopterin mutant enzyme H35N, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 0.07
-
7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 25°C Methanocaldococcus jannaschii
4.1.2.25 0.64
-
7,8-dihydroneopterin mutant enzyme Y111F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 0.68
-
7,8-dihydroneopterin mutant enzyme E25Q, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 1
-
7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 70°C Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.25 7.5 8.5
-
Methanocaldococcus jannaschii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.25 10.5
-
there is no protein aggregation or precipitation observed when proteins are incubated for 10 min at 70°C in 25mM tricine-CAPS-TES buffer at pH 10.5 Methanocaldococcus jannaschii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.25 1.1
-
7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 25°C Methanocaldococcus jannaschii
4.1.2.25 1.8
-
7,8-dihydroneopterin mutant enzyme Y78F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 2.6
-
7,8-dihydroneopterin mutant enzyme H35N, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 2.7
-
7,8-dihydroneopterin Km below 2.7 1/sec*mM, mutant enzyme E25Q, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 5.7
-
7,8-dihydroneopterin mutant enzyme Q61A, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 58
-
7,8-dihydroneopterin mutant enzyme Y111F, at pH 7.6 and 70°C Methanocaldococcus jannaschii
4.1.2.25 100
-
7,8-dihydroneopterin wild type enzyme, at pH 7.6 and 70°C Methanocaldococcus jannaschii