Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Irwin, D.; Shin,D.-H.; Zhang, S.; Barr,b.K.; Sakon,j.; Karplus, P.A.; Wilson, D.B.
    Roles of the catalytic domain and two cellulose binding domains of Thermomonospora fusca E4 in cellulose hydrolysis (1998), J. Bacteriol., 180, 1709-1714 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B42 expression in Streptomyces lividans and Escherichia coli Thermobifida fusca

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.B42 D55C mutation in proposed catalytic site, low activity, mutant is able to bind bacterial microcrystalline cellulose Thermobifida fusca
3.2.1.B42 additional information Removal of the internal family IIIc CBD decreases activity markedly on every substrate. Constructs which lack the family IIIc CBD have almost no activity on bacterial microcrystalline cellulose Thermobifida fusca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B42 90400
-
 calculated from sequence Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B42 Thermobifida fusca P26221
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B42 carboxymethyl cellulose + H2O
-
 Thermobifida fusca cellotetraose + ?
-
 ?
3.2.1.B42 cellulose + H2O bacterial microcrystalline cellulose Thermobifida fusca cellotetraose + ?
-
 ?
3.2.1.B42 filter paper + H2O
-
 Thermobifida fusca cellotetraose + ?
-
 ?
3.2.1.B42 swollen cellulose paper + H2O
-
 Thermobifida fusca cellotetraose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B42 ? x * 90400, calculated from sequence Thermobifida fusca

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B42 CelD
-
 Thermobifida fusca
3.2.1.B42 E4
-
 Thermobifida fusca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B42 56
-
 16 h, 50% of the activity is retained for constructions lacking the type IIIc CBD Thermobifida fusca
3.2.1.B42 63
-
 16 h, 50% of the activity is retained for constructions containing the type IIIc CBD Thermobifida fusca

General Information

EC Number General Information Comment Organism
3.2.1.B42 physiological function enzyme is comprised of a catalytic domain (CD), an internal family IIIc cellulose binding domain (CBD), a fibronectinlike domain, and a family II CBD. Removal of the internal family IIIc CBD decreases activity markedly on every substrate Thermobifida fusca