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Literature summary extracted from

  • Kotsuka, T.; Akanuma, S.; Tomuro, M.; Yamagishi, A.; Oshima, T.
    Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method (1996), J. Bacteriol., 178, 723-727 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.85 A172V mutation improves thermal stability, without significantly changing its catalytic properties Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.022
-
 (2R,3S)-3-isopropylmalate 60°C, pH 7.6, mutant enzyme A172V Thermus thermophilus
1.1.1.85 0.031
-
 (2R,3S)-3-isopropylmalate 60°C, pH 7.6, wild-type enzyme Thermus thermophilus
1.1.1.85 0.13
-
 NAD+ 60°C, pH 7.6, pH 7.6, mutant enzyme A172V Thermus thermophilus
1.1.1.85 0.14
-
 NAD+ 60°C, pH 7.6, wild-type enzyme Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Thermus thermophilus P61494
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.85
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Thermus thermophilus (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.85 dimer
-
 Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.85 IPMDH
-
 Thermus thermophilus
1.1.1.85 leuB
-
 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.85 163
-
 NAD+ 60°C, pH 7.6, wild-type enzyme Thermus thermophilus
1.1.1.85 163
-
 (2R,3S)-3-isopropylmalate 60°C, pH 7.6, wild-type enzyme Thermus thermophilus
1.1.1.85 220
-
 (2R,3S)-3-isopropylmalate 60°C, pH 7.6, mutant enzyme A172V Thermus thermophilus
1.1.1.85 220
-
 NAD+ 60°C, pH 7.6, pH 7.6, mutant enzyme A172V Thermus thermophilus