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Literature summary extracted from

  • Chou, W.K.; Gould, C.A.; Cane, D.E.
    Incubation of 2-methylisoborneol synthase with the intermediate analog 2-methylneryl diphosphate (2017), J. Antibiot., 70, 625-631 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.118 gene sco7700, recombinant overexpression of His-tagged enzyme Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.118 additional information
-
 additional information Michaelis-Menten steady-state kinetics Streptomyces coelicolor
4.2.3.118 0.018
-
 2-methylneryl diphosphate pH 6.7, 30°C Streptomyces coelicolor
4.2.3.118 0.095
-
 (E)-2-methylgeranyl diphosphate pH 6.7, 30°C Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.118 (E)-2-methylgeranyl diphosphate + H2O Streptomyces coelicolor
-
 2-methylisoborneol + diphosphate
-
 ?
4.2.3.118 (E)-2-methylgeranyl diphosphate + H2O Streptomyces coelicolor ATCC BAA-471 / A3(2) / M145
-
 2-methylisoborneol + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.118 Streptomyces coelicolor Q9F1Y6
-
-
4.2.3.118 Streptomyces coelicolor ATCC BAA-471 / A3(2) / M145 Q9F1Y6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.118 recombinant His-tagged enzyme by nickel affinity chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.118 (E)-2-methylgeranyl diphosphate + H2O
-
 Streptomyces coelicolor 2-methylisoborneol + diphosphate
-
 ?
4.2.3.118 (E)-2-methylgeranyl diphosphate + H2O
-
 Streptomyces coelicolor ATCC BAA-471 / A3(2) / M145 2-methylisoborneol + diphosphate
-
 ?
4.2.3.118 2-methylneryl diphosphate + H2O
-
 Streptomyces coelicolor 2-methyllimonene
-
 ?
4.2.3.118 2-methylneryl diphosphate + H2O
-
 Streptomyces coelicolor ATCC BAA-471 / A3(2) / M145 2-methyllimonene
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.118 MIBS
-
 Streptomyces coelicolor
4.2.3.118 sco7700
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.118 30
-
 assay at Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.118 0.0046
-
 2-methylneryl diphosphate pH 6.7, 30°C Streptomyces coelicolor
4.2.3.118 0.105
-
 (E)-2-methylgeranyl diphosphate pH 6.7, 30°C Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.118 6.7
-
 assay at Streptomyces coelicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.118 0.256
-
 2-methylneryl diphosphate pH 6.7, 30°C Streptomyces coelicolor
4.2.3.118 1.105
-
 (E)-2-methylgeranyl diphosphate pH 6.7, 30°C Streptomyces coelicolor