EC Number | Application | Comment | Organism |
---|---|---|---|
5.1.3.30 | synthesis | D-psicose 3-epimerase is the key enzyme for producing D-allulose from inexpensive D-fructose sugar | [Clostridium] scindens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.3.30 | recombinant expression of D-psicose 3-epimerase and display on the surface of Bacillus subtilis spores. To improve the expression level of D-psicose 3-epimerase, the DPEase gene is fused with the promoter P43 to generate an expression cassette that introduced an identical cohesive end, and the resultant P43-DPE expression cassette is used as a monomeric fragment. The tandem repeats of the expression cassette are constructed by the isocaudamer method and integrated into the amyE gene locus of Bacillus subtilis by double-crossover. After DPEase is expressed in Bacillus subtilis, the antibiotic resistance gene is deleted via the Cre/lox system, thus generating food-grade strains | [Clostridium] scindens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.1.3.30 | additional information | to improve the expression level of D-psicose 3-epimerase, the DPEase gene is fused with the promoter P43 to generate an expression cassette that introduced an identical cohesive end, and the resultant P43-DPE expression cassette is used as a monomeric fragment. The tandem repeats of the expression cassette are constructed by the isocaudamer method and integrated into the amyE gene locus of Bacillus subtilis by double-crossover. After DPEase is expressed in Bacillus subtilis, the antibiotic resistance gene is deleted via the Cre/lox system, thus generating food-grade strains. D-Psicose (D-allulose) production by freeze-dried enzyme powder, the optimal substrate concentration is 300 g/L of D-fructose, generating 26.67 g/l/h of D-allulose yield with an 8.89% conversion rate | [Clostridium] scindens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.3.30 | D-psicose | [Clostridium] scindens | - |
D-fructose | - |
r | |
5.1.3.30 | D-psicose | [Clostridium] scindens ATCC 35704 | - |
D-fructose | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.3.30 | [Clostridium] scindens | - |
- |
- |
5.1.3.30 | [Clostridium] scindens ATCC 35704 | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.3.30 | D-psicose | - |
[Clostridium] scindens | D-fructose | - |
r | |
5.1.3.30 | D-psicose | - |
[Clostridium] scindens ATCC 35704 | D-fructose | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.3.30 | DPEase | - |
[Clostridium] scindens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.3.30 | 60 | - |
assay at | [Clostridium] scindens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.3.30 | 7.5 | - |
assay at | [Clostridium] scindens |