BRENDA - Enzyme Database

Cloning and characterization of surface-localized alpha-enolase of Streptococcus iniae, an effective protective antigen in mice

Wang, J.; Wang, K.; Chen, D.; Geng, Y.; Huang, X.; He, Y.; Ji, L.; Liu, T.; Wang, E.; Yang, Q.; Lai, W.; Int. J. Mol. Sci. 16, 14490-14510 (2015)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
4.2.1.11
medicine
recombinant alpha-enolase can confer effective protection against Streptococcus iniae infection in mice
Streptococcus iniae
Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli
Streptococcus iniae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
1.5
2
2-phospho-D-glycerate
pH 7.0, temperature not specified in the publication
Streptococcus iniae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Streptococcus iniae
T1TFA0
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
747973
Streptococcus iniae
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
phosphoenolpyruvate + H2O
-
747973
Streptococcus iniae
2-phospho-D-glycerate
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47240, calculated from sequence, x * 67000, SDS-PAGE of recombinant protein with His-tag
Streptococcus iniae
Application (protein specific)
EC Number
Application
Commentary
Organism
4.2.1.11
medicine
recombinant alpha-enolase can confer effective protection against Streptococcus iniae infection in mice
Streptococcus iniae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli
Streptococcus iniae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
1.5
2
2-phospho-D-glycerate
pH 7.0, temperature not specified in the publication
Streptococcus iniae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
747973
Streptococcus iniae
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
phosphoenolpyruvate + H2O
-
747973
Streptococcus iniae
2-phospho-D-glycerate
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47240, calculated from sequence, x * 67000, SDS-PAGE of recombinant protein with His-tag
Streptococcus iniae
General Information
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
enzyme interacts with human plasminogen and participates in Streptococcus iniae adhesion to and invasion of BHK-21 cells
Streptococcus iniae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
enzyme interacts with human plasminogen and participates in Streptococcus iniae adhesion to and invasion of BHK-21 cells
Streptococcus iniae