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Literature summary extracted from

  • Zhu, Y.; Yu, S.; Huang, D.; Zhang, T.; Jiang, B.; Mu, W.
    Identification of a novel DFA I-producing inulin fructotransferase from Streptomyces davawensis (2016), Int. J. Biol. Macromol., 92, 723-730 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.17 expression in Escherichia coli Streptomyces davaonensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.17 homology modelling and molecular docking. Residues D183 and E194 are potential catalytic residues Streptomyces davaonensis

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.17 D183A complete loss of activity Streptomyces davaonensis
4.2.2.17 D183N complete loss of activity Streptomyces davaonensis
4.2.2.17 E194A complete loss of activity Streptomyces davaonensis
4.2.2.17 E194N complete loss of activity Streptomyces davaonensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.17 2.89
-
 inulin pH 5.5, 40°C Streptomyces davaonensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.17 12500
-
 gel fitration Streptomyces davaonensis

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.17 Streptomyces davaonensis
-
-
-
4.2.2.17 Streptomyces davaonensis SK39.001
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.17 fructofuranosyl nystose
-
 Streptomyces davaonensis di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
 ?
4.2.2.17 fructofuranosyl nystose
-
 Streptomyces davaonensis SK39.001 di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
 ?
4.2.2.17 inulin
-
 Streptomyces davaonensis di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
-
 ?
4.2.2.17 inulin
-
 Streptomyces davaonensis SK39.001 di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
-
 ?
4.2.2.17 additional information no substrate: 1-kestose Streptomyces davaonensis ?
-
 ?
4.2.2.17 additional information no substrate: 1-kestose Streptomyces davaonensis SK39.001 ?
-
 ?
4.2.2.17 nystose
-
 Streptomyces davaonensis di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
 ?
4.2.2.17 nystose
-
 Streptomyces davaonensis SK39.001 di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.17 trimer 3 * 40000, SDS-PAGE, 3 * 40769, calculated from sequence Streptomyces davaonensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.17 40
-
-
 Streptomyces davaonensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.17 70
-
 2h, 40% residual activity Streptomyces davaonensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.17 5.5
-
-
 Streptomyces davaonensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.17 6 6.5 more than 69% of maximum activity Streptomyces davaonensis