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Literature summary extracted from

  • Rani, A.; Dhillon, A.; Sharma, K.; Goyal, A.
    Insights into the structural characteristics and substrate binding analysis of chondroitin AC lyase (PsPL8A) from Pedobacter saltans (2018), Int. J. Biol. Macromol., 109, 980-991 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.5 modeling of structure. Residues N153, W105, H203, Y208, Y212, R266 and E349 are involved in catalysis Pseudopedobacter saltans

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.5 E349A no residual activity Pseudopedobacter saltans
4.2.2.5 H203A no residual activity Pseudopedobacter saltans
4.2.2.5 N153A no residual activity Pseudopedobacter saltans
4.2.2.5 R266A no residual activity Pseudopedobacter saltans
4.2.2.5 Y212F no residual activity Pseudopedobacter saltans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.5 EDTA presence of EDTA decreases the melting temperature Pseudopedobacter saltans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.5 Ca2+ presence of Ca2+ enhances the enzyme activity but does not impart any structural stability Pseudopedobacter saltans

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.5 Pseudopedobacter saltans F0S764
-
-
4.2.2.5 Pseudopedobacter saltans DSM 12145 F0S764
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.5 proteolytic modification sequence contains a N-terminal signal peptide with a cleavage site between Ala22 and Gln23 Pseudopedobacter saltans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.5 chondroitin 4-sulfate
-
 Pseudopedobacter saltans ?
-
 ?
4.2.2.5 chondroitin 4-sulfate
-
 Pseudopedobacter saltans DSM 12145 ?
-
 ?
4.2.2.5 chondroitin 6-sulfate
-
 Pseudopedobacter saltans ?
-
 ?
4.2.2.5 chondroitin 6-sulfate
-
 Pseudopedobacter saltans DSM 12145 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.5 Pedsa_3808
-
 Pseudopedobacter saltans
4.2.2.5 PL8A
-
 Pseudopedobacter saltans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.5 52
-
 melting temperature in presence of 5 mM EDTA Pseudopedobacter saltans
4.2.2.5 60
-
 protein starts unfolding at 45°C and it completely melts at 60°C Pseudopedobacter saltans