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Literature summary extracted from

  • Dumont, S.; Bykova, N.; Pelletier, G.; Dorion, S.; Rivoal, J.
    Cytosolic triosephosphate isomerase from Arabidopsis thaliana is reversibly modified by glutathione on cysteines 127 and 218 (2016), Front. Plant Sci., 7, 1942 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.1 expression in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.1 C127S loss of more than 97% of specific activity Arabidopsis thaliana
5.3.1.1 C127S/C218S loss of more than 97% of specific activity Arabidopsis thaliana
5.3.1.1 C218S loss of about 21% of specific activity. In presence of glutathione disulfide, mutant behaves similarly to the wild-type, but retains more activity than the wild-type after 240 min of incubation Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.1 Diamide 1 mM, presence of glutathione, complete inhibition by S-glutathionylation Arabidopsis thaliana
5.3.1.1 glutathione
-
 Arabidopsis thaliana
5.3.1.1 glutathione disulfide modification of residues Cys127 and Cys218 Arabidopsis thaliana
5.3.1.1 H2O2 0.5 mM, 120 min, 30% loss of activity in presence and absence of glutathione Arabidopsis thaliana
5.3.1.1 N-ethylmaleimide 0.5 mM, 60 min, more than 90% loss of activity. Addition of dithiothreitol does not restore activity. In the presence of 1 mM glutathione, the addition of 0.5 mM N-ethylmaleimide does not significantly affect activity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.1 0.48
-
 D-glyceraldehyde 3-phosphate mutant C127S, pH 7.8, 30°C Arabidopsis thaliana
5.3.1.1 0.5
-
 D-glyceraldehyde 3-phosphate mutant C127S/C218S, pH 7.8, 30°C Arabidopsis thaliana
5.3.1.1 1.32
-
 D-glyceraldehyde 3-phosphate wild-type, pH 7.8, 30°C Arabidopsis thaliana
5.3.1.1 1.68
-
 D-glyceraldehyde 3-phosphate mutant C218S, pH 7.8, 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.1.1 cytosol
-
 Arabidopsis thaliana 5829
-

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.1 Arabidopsis thaliana P48491
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.1 D-glyceraldehyde 3-phosphate
-
 Arabidopsis thaliana dihydroxyacetone 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.1 CTIMC
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.1 14.8
-
 D-glyceraldehyde 3-phosphate mutant C127S/C218S, pH 7.8, 30°C Arabidopsis thaliana
5.3.1.1 70.4
-
 D-glyceraldehyde 3-phosphate mutant C127S, pH 7.8, 30°C Arabidopsis thaliana
5.3.1.1 2690
-
 D-glyceraldehyde 3-phosphate mutant C218S, pH 7.8, 30°C Arabidopsis thaliana
5.3.1.1 3406
-
 D-glyceraldehyde 3-phosphate wild-type, pH 7.8, 30°C Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.1 29.6
-
 D-glyceraldehyde 3-phosphate mutant C127S/C218S, pH 7.8, 30°C Arabidopsis thaliana
5.3.1.1 147
-
 D-glyceraldehyde 3-phosphate mutant C127S, pH 7.8, 30°C Arabidopsis thaliana
5.3.1.1 1601
-
 D-glyceraldehyde 3-phosphate mutant C218S, pH 7.8, 30°C Arabidopsis thaliana
5.3.1.1 2580
-
 D-glyceraldehyde 3-phosphate wild-type, pH 7.8, 30°C Arabidopsis thaliana