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Literature summary extracted from

  • Katano, M.; Takahashi, K.; Hirano, T.; Kazama, Y.; Abe, T.; Tsukaya, H.; Ferjani, A.
    Suppressor screen and phenotype analyses revealed an emerging role of the monofunctional peroxisomal enoyl-CoA hydratase 2 in compensated cell enlargement (2016), Front. Plant Sci., 7, 132 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.119 gene ECH2, DNA and amino acid sequence determination and analysis, map-based cloning Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.119 additional information mutagenesis of fugu5-1 seeds with heavy-ion irradiation and screening of mutations that restrain compensated cell enlargement (CCE) to gain insight into the genetic pathway(s) involved in CCE. Mutant A#3-1 has a cell size that is severely reduced, but the cell number remains similar to that of original fugu5-1. The cell number decreases in A#3-1 single mutant (A#3-1sm), similar to that of fugu5-1, but cell size is almost equal to that of the wild-type. A#3-1 mutation does not affect CCE in other compensation exhibiting mutant backgrounds, such as an3-4 and fugu2-1/fas1-6. Subsequent map-based cloning combined with genome sequencing and HRM curve analysis identified enoyl-CoA hydratase 2 (ECH2) as the causal gene of A#3-1. The above phenotypes are consistently observed in the ech2-1 allele and supplying sucrose restores the morphological and cellular phenotypes in fugu5-1, ech2-1, A#3-1sm, fugu5-1 ech2-1, and A#3-1; fugu5-1. The ech2-1 mutant allele is indistinguishable from A#3-1sm and suppresses CCEin fugu5. Analysis of ech2-1 mutant. Enoyl-CoA hydratase 2 gene mutation causes the A#3-1sm phenotypes, overview Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.119 peroxisome
-
Arabidopsis thaliana 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.119 (2E)-2-enoyl-CoA + H2O Arabidopsis thaliana
-
(3R)-3-hydroxyacyl-CoA
-
r
4.2.1.119 (2E)-2-enoyl-CoA + H2O Arabidopsis thaliana Col-0
-
(3R)-3-hydroxyacyl-CoA
-
r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.119 Arabidopsis thaliana Q8VYI3 mutant fugu5-1
-
4.2.1.119 Arabidopsis thaliana Col-0 Q8VYI3 mutant fugu5-1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.119 cotyledon
-
Arabidopsis thaliana
-
4.2.1.119 leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.119 (2E)-2-enoyl-CoA + H2O
-
Arabidopsis thaliana (3R)-3-hydroxyacyl-CoA
-
r
4.2.1.119 (2E)-2-enoyl-CoA + H2O
-
Arabidopsis thaliana Col-0 (3R)-3-hydroxyacyl-CoA
-
r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.119 At1g76150
-
Arabidopsis thaliana
4.2.1.119 ECH2
-
Arabidopsis thaliana
4.2.1.119 peroxisomal enoyl-CoA hydratase 2
-
Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
4.2.1.119 malfunction defects in either HC-PPase or ECH2 compromise cell proliferation due to defects in mobilizing seed storage lipids, phenotype, overview. Enoyl-CoA hydratase 2 (ECH2) gene mutation causes the A#3-1sm phenotypes, overview. Mutant A#3-1 has a cell size that is severely reduced, but the cell number remains similar to that of original fugu5-1. The cell number decreases in A#3-1 single mutant (A#3-1sm), similar to that of fugu5-1, but cell size is almost equal to that of the wild-type. A#3-1 mutation does not affect CCE in other compensation exhibiting mutant backgrounds, such as an3-4 and fugu2-1/fas1-6 Arabidopsis thaliana
4.2.1.119 physiological function role of the monofunctional peroxisomal enoyl-CoA hydratase 2 in compensated cell enlargement (CCE). Enzyme ECH2 alone likely promotes CCE during the post-mitotic cell expansion stage of cotyledon development, probably by converting indolebutyric acid to indole acetic acid Arabidopsis thaliana