EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.119 | gene ECH2, DNA and amino acid sequence determination and analysis, map-based cloning | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.119 | additional information | mutagenesis of fugu5-1 seeds with heavy-ion irradiation and screening of mutations that restrain compensated cell enlargement (CCE) to gain insight into the genetic pathway(s) involved in CCE. Mutant A#3-1 has a cell size that is severely reduced, but the cell number remains similar to that of original fugu5-1. The cell number decreases in A#3-1 single mutant (A#3-1sm), similar to that of fugu5-1, but cell size is almost equal to that of the wild-type. A#3-1 mutation does not affect CCE in other compensation exhibiting mutant backgrounds, such as an3-4 and fugu2-1/fas1-6. Subsequent map-based cloning combined with genome sequencing and HRM curve analysis identified enoyl-CoA hydratase 2 (ECH2) as the causal gene of A#3-1. The above phenotypes are consistently observed in the ech2-1 allele and supplying sucrose restores the morphological and cellular phenotypes in fugu5-1, ech2-1, A#3-1sm, fugu5-1 ech2-1, and A#3-1; fugu5-1. The ech2-1 mutant allele is indistinguishable from A#3-1sm and suppresses CCEin fugu5. Analysis of ech2-1 mutant. Enoyl-CoA hydratase 2 gene mutation causes the A#3-1sm phenotypes, overview | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.119 | peroxisome | - |
Arabidopsis thaliana | 5777 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.119 | (2E)-2-enoyl-CoA + H2O | Arabidopsis thaliana | - |
(3R)-3-hydroxyacyl-CoA | - |
r | |
4.2.1.119 | (2E)-2-enoyl-CoA + H2O | Arabidopsis thaliana Col-0 | - |
(3R)-3-hydroxyacyl-CoA | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.119 | Arabidopsis thaliana | Q8VYI3 | mutant fugu5-1 | - |
4.2.1.119 | Arabidopsis thaliana Col-0 | Q8VYI3 | mutant fugu5-1 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.1.119 | cotyledon | - |
Arabidopsis thaliana | - |
4.2.1.119 | leaf | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.119 | (2E)-2-enoyl-CoA + H2O | - |
Arabidopsis thaliana | (3R)-3-hydroxyacyl-CoA | - |
r | |
4.2.1.119 | (2E)-2-enoyl-CoA + H2O | - |
Arabidopsis thaliana Col-0 | (3R)-3-hydroxyacyl-CoA | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.119 | At1g76150 | - |
Arabidopsis thaliana |
4.2.1.119 | ECH2 | - |
Arabidopsis thaliana |
4.2.1.119 | peroxisomal enoyl-CoA hydratase 2 | - |
Arabidopsis thaliana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.119 | malfunction | defects in either HC-PPase or ECH2 compromise cell proliferation due to defects in mobilizing seed storage lipids, phenotype, overview. Enoyl-CoA hydratase 2 (ECH2) gene mutation causes the A#3-1sm phenotypes, overview. Mutant A#3-1 has a cell size that is severely reduced, but the cell number remains similar to that of original fugu5-1. The cell number decreases in A#3-1 single mutant (A#3-1sm), similar to that of fugu5-1, but cell size is almost equal to that of the wild-type. A#3-1 mutation does not affect CCE in other compensation exhibiting mutant backgrounds, such as an3-4 and fugu2-1/fas1-6 | Arabidopsis thaliana |
4.2.1.119 | physiological function | role of the monofunctional peroxisomal enoyl-CoA hydratase 2 in compensated cell enlargement (CCE). Enzyme ECH2 alone likely promotes CCE during the post-mitotic cell expansion stage of cotyledon development, probably by converting indolebutyric acid to indole acetic acid | Arabidopsis thaliana |