EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.3.2.10 | N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide | binding of the allosteric effector ligand stimulates millisecond timescale motions in the enzyme that enhance its catalytic function. The flexibility of the apo enzyme is nearly identical to that of its N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide activated state at 70°C, whereas conformational motions are considerably different between the two forms of the enzyme at room temperature. Allosteric activation by N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide decreases to 65fold at 70°C, compared to 4200fold at 30°C | Thermotoga maritima |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.2.10 | - |
Thermotoga maritima |
4.3.2.10 | expression in Escherichia coli BL21(DE3) | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.2.10 | 1.3 | - |
L-glutamine | pH 8.0, 30°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 1.32 | - |
L-glutamine | pH 8.0, 70°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 1.35 | - |
L-glutamine | pH 8.0, 40°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 1.36 | - |
L-glutamine | pH 8.0, 60°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 1.64 | - |
L-glutamine | pH 8.0, 50°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 2.96 | - |
L-glutamine | pH 8.0, 70°C | Thermotoga maritima | |
4.3.2.10 | 2.96 | - |
L-glutamine | pH 8.0, 70°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 3.8 | - |
L-glutamine | pH 8.0, 50°C | Thermotoga maritima | |
4.3.2.10 | 3.8 | - |
L-glutamine | pH 8.0, 50°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 3.89 | - |
L-glutamine | pH 8.0, 60°C | Thermotoga maritima | |
4.3.2.10 | 3.89 | - |
L-glutamine | pH 8.0, 60°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 4.71 | - |
L-glutamine | pH 8.0, 40°C | Thermotoga maritima | |
4.3.2.10 | 4.71 | - |
L-glutamine | pH 8.0, 40°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 4.91 | - |
L-glutamine | pH 8.0, 30°C | Thermotoga maritima | |
4.3.2.10 | 4.91 | - |
L-glutamine | pH 8.0, 30°C, basal activity | Thermotoga maritima |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.2.10 | 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | Thermotoga maritima | the enzyme is involved in histidine biosynthesis | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? | |
4.3.2.10 | 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | Thermotoga maritima ATCC 43589 | the enzyme is involved in histidine biosynthesis | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.2.10 | Thermotoga maritima | Q9X0C6 AND Q9X0C8 | Q9X0C6: subunit HisF, Q9X0C8: subunit HisH | - |
4.3.2.10 | Thermotoga maritima | Q9X0C8 and Q9X0C6 | Q9X0C8: subunit HisH, Q9X0C6: subunit HisF | - |
4.3.2.10 | Thermotoga maritima ATCC 43589 | Q9X0C6 AND Q9X0C8 | Q9X0C6: subunit HisF, Q9X0C8: subunit HisH | - |
4.3.2.10 | Thermotoga maritima ATCC 43589 | Q9X0C8 and Q9X0C6 | Q9X0C8: subunit HisH, Q9X0C6: subunit HisF | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.2.10 | - |
Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.2.10 | 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | - |
Thermotoga maritima | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? | |
4.3.2.10 | 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | the enzyme is involved in histidine biosynthesis | Thermotoga maritima | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? | |
4.3.2.10 | 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | - |
Thermotoga maritima ATCC 43589 | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? | |
4.3.2.10 | 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine | the enzyme is involved in histidine biosynthesis | Thermotoga maritima ATCC 43589 | 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate | - |
? | |
4.3.2.10 | L-glutamine + H2O | - |
Thermotoga maritima | L-glutamate + NH3 | - |
? | |
4.3.2.10 | L-glutamine + H2O | binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70°C, compared to 4200-fold at 30°C | Thermotoga maritima | L-glutamate + NH3 | - |
? | |
4.3.2.10 | L-glutamine + H2O | binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70°C, compared to 4200-fold at 30°C | Thermotoga maritima ATCC 43589 | L-glutamate + NH3 | - |
? | |
4.3.2.10 | L-glutamine + H2O | - |
Thermotoga maritima ATCC 43589 | L-glutamate + NH3 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.2.10 | gps | - |
Thermotoga maritima |
4.3.2.10 | IGPS | - |
Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.2.10 | 30 | - |
assay at | Thermotoga maritima |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.2.10 | 30 | 70 | binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70°C, compared to 4200-fold at 30°C | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.2.10 | 0.00372 | - |
L-glutamine | pH 8.0, 30°C | Thermotoga maritima | |
4.3.2.10 | 0.00372 | - |
L-glutamine | pH 8.0, 30°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 0.0571 | - |
L-glutamine | pH 8.0, 40°C | Thermotoga maritima | |
4.3.2.10 | 0.0571 | - |
L-glutamine | pH 8.0, 40°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 0.139 | - |
L-glutamine | pH 8.0, 50°C | Thermotoga maritima | |
4.3.2.10 | 0.139 | - |
L-glutamine | pH 8.0, 50°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 0.204 | - |
L-glutamine | pH 8.0, 70°C | Thermotoga maritima | |
4.3.2.10 | 0.204 | - |
L-glutamine | pH 8.0, 70°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 0.212 | - |
L-glutamine | pH 8.0, 60°C | Thermotoga maritima | |
4.3.2.10 | 0.212 | - |
L-glutamine | pH 8.0, 60°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 4.09 | - |
L-glutamine | pH 8.0, 30°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 4.37 | - |
L-glutamine | pH 8.0, 40°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 4.89 | - |
L-glutamine | pH 8.0, 50°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 5.5 | - |
L-glutamine | pH 8.0, 60°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 5.92 | - |
L-glutamine | pH 8.0, 70°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.2.10 | 8 | - |
assay at | Thermotoga maritima |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.3.2.10 | metabolism | the enzyme is involved in histidine biosynthesis | Thermotoga maritima |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.2.10 | 0.000758 | - |
L-glutamine | pH 8.0, 30°C | Thermotoga maritima | |
4.3.2.10 | 0.0121 | - |
L-glutamine | pH 8.0, 40°C | Thermotoga maritima | |
4.3.2.10 | 0.0365 | - |
L-glutamine | pH 8.0, 50°C | Thermotoga maritima | |
4.3.2.10 | 0.054 | - |
L-glutamine | pH 8.0, 60°C | Thermotoga maritima | |
4.3.2.10 | 0.0689 | - |
L-glutamine | pH 8.0, 70°C | Thermotoga maritima | |
4.3.2.10 | 0.121 | - |
L-glutamine | pH 8.0, 40°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 0.365 | - |
L-glutamine | pH 8.0, 50°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 0.544 | - |
L-glutamine | pH 8.0, 60°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 0.688 | - |
L-glutamine | pH 8.0, 70°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 0.758 | - |
L-glutamine | pH 8.0, 30°C, basal activity | Thermotoga maritima | |
4.3.2.10 | 2980 | - |
L-glutamine | pH 8.0, 50°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 3150 | - |
L-glutamine | pH 8.0, 30°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 3240 | - |
L-glutamine | pH 8.0, 40°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 4040 | - |
L-glutamine | pH 8.0, 60°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima | |
4.3.2.10 | 4480 | - |
L-glutamine | pH 8.0, 70°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide | Thermotoga maritima |