BRENDA - Enzyme Database

Biochemical and structural characterization of enolase from Chloroflexus aurantiacus Evidence for a thermophilic origin

Zadvornyy, O.A.; Boyd, E.S.; Posewitz, M.C.; Zorin, N.A.; Peters, J.W.; Front. Bioeng. Biotechnol. 3, 74 (2015)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.03
-
2-phospho-D-glycerate
pH 6.5, 80°C
Chloroflexus aurantiacus
4.2.1.11
0.16
-
2-phospho-D-glycerate
pH 6.5, 25°C
Chloroflexus aurantiacus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
Km value 2.5 mM at 25°C, 1.9 mM at 80°C, respectively
Chloroflexus aurantiacus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Chloroflexus aurantiacus
A9WCM4
-
-
4.2.1.11
Chloroflexus aurantiacus DSM 635
A9WCM4
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
-
Chloroflexus aurantiacus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
147
-
pH 6.5, 25°C
Chloroflexus aurantiacus
4.2.1.11
300
-
pH 6.5, 80°C
Chloroflexus aurantiacus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
747831
Chloroflexus aurantiacus
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
747831
Chloroflexus aurantiacus DSM 635
phosphoenolpyruvate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 46000, SDS-PAGE
Chloroflexus aurantiacus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
80
-
-
Chloroflexus aurantiacus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.5
-
-
Chloroflexus aurantiacus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.03
-
2-phospho-D-glycerate
pH 6.5, 80°C
Chloroflexus aurantiacus
4.2.1.11
0.16
-
2-phospho-D-glycerate
pH 6.5, 25°C
Chloroflexus aurantiacus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
Km value 2.5 mM at 25°C, 1.9 mM at 80°C, respectively
Chloroflexus aurantiacus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
-
Chloroflexus aurantiacus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
147
-
pH 6.5, 25°C
Chloroflexus aurantiacus
4.2.1.11
300
-
pH 6.5, 80°C
Chloroflexus aurantiacus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
747831
Chloroflexus aurantiacus
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
747831
Chloroflexus aurantiacus DSM 635
phosphoenolpyruvate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
2 * 46000, SDS-PAGE
Chloroflexus aurantiacus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
80
-
-
Chloroflexus aurantiacus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.5
-
-
Chloroflexus aurantiacus