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Literature summary extracted from

  • Zadvornyy, O.A.; Boyd, E.S.; Posewitz, M.C.; Zorin, N.A.; Peters, J.W.
    Biochemical and structural characterization of enolase from Chloroflexus aurantiacus Evidence for a thermophilic origin (2015), Front. Bioeng. Biotechnol., 3, 74 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.03
-
 2-phospho-D-glycerate pH 6.5, 80°C Chloroflexus aurantiacus
4.2.1.11 0.16
-
 2-phospho-D-glycerate pH 6.5, 25°C Chloroflexus aurantiacus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 Mg2+ Km value 2.5 mM at 25°C, 1.9 mM at 80°C, respectively Chloroflexus aurantiacus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Chloroflexus aurantiacus A9WCM4
-
-
4.2.1.11 Chloroflexus aurantiacus DSM 635 A9WCM4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11
-
 Chloroflexus aurantiacus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 147
-
 pH 6.5, 25°C Chloroflexus aurantiacus
4.2.1.11 300
-
 pH 6.5, 80°C Chloroflexus aurantiacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Chloroflexus aurantiacus phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 2-phospho-D-glycerate
-
 Chloroflexus aurantiacus DSM 635 phosphoenolpyruvate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.11 dimer 2 * 46000, SDS-PAGE Chloroflexus aurantiacus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 ENO
-
 Chloroflexus aurantiacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.11 80
-
-
 Chloroflexus aurantiacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.11 6.5
-
-
 Chloroflexus aurantiacus